In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue

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dc.contributor.authorChisholm, K. I.
dc.contributor.authorIda, K. K.
dc.contributor.authorDavies, A. L.
dc.contributor.authorPapkovsky, Dmitri B.
dc.contributor.authorSinger, M.
dc.contributor.authorDyson, A.
dc.contributor.authorTachtsidis, I.
dc.contributor.authorDuchen, M. R.
dc.contributor.authorSmith, K. J.
dc.contributor.funderWellcome Trusten
dc.contributor.funderUniversity College Londonen
dc.contributor.funderMultiple Sclerosis Societyen
dc.date.accessioned2019-10-25T09:05:34Z
dc.date.available2019-10-25T09:05:34Z
dc.date.issued2016-01
dc.date.updated2019-10-25T08:37:35Z
dc.description.abstractLive imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have observed flavoprotein fluorescence in the exposed murine cerebral cortex in vivo using confocal imaging; the mitochondrial origin of the signal was confirmed using agents known to manipulate mitochondrial redox potential. The effects of cerebral oxygenation on flavoprotein fluorescence were determined by manipulating the inspired oxygen concentration. We report that flavoprotein fluorescence is sensitive to reductions in cortical oxygenation, such that reductions in inspired oxygen resulted in loss of flavoprotein fluorescence with the exception of a preserved ‘halo’ of signal in periarterial regions. The findings are consistent with reports that arteries play an important role in supplying oxygen directly to tissue in the cerebral cortex, maintaining mitochondrial function.en
dc.description.sponsorshipUniversity College London (Grand Challenges)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationChisholm, K. I., Ida, K. K., Davies, A. L., Papkovsky, D. B., Singer, M., Dyson, A., Tachtsidis, I., Duchen, M. R. and Smith, K. J. (2016) 'In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue', in Elwell, C. E., Leung, T. S. and Harrison, D. K. (eds) Oxygen Transport to Tissue XXXVII. Advances in Experimental Medicine and Biology, vol 876. Springer, New York, NY, pp. 233-239. doi: 10.1007/978-1-4939-3023-4_29en
dc.identifier.doi10.1007/978-1-4939-3023-4_29en
dc.identifier.endpage239en
dc.identifier.isbn978-1-4939-3022-7
dc.identifier.isbn978-1-4939-3023-4
dc.identifier.journaltitleAdvances in Experimental Medicine and Biologyen
dc.identifier.startpage233en
dc.identifier.urihttps://hdl.handle.net/10468/8857
dc.identifier.volume876en
dc.language.isoenen
dc.publisherSpringer Nature Switzerland AGen
dc.relation.ispartofAdvances in Experimental Medicine and Biology book series (AEMB, volume 876)
dc.relation.urihttp://www.isott.org/index.php/news-reader/items/isott-2014-london-uk.html
dc.relation.urihttps://doi.org/10.1007/978-1-4939-3023-4_66
dc.rights© 2016, the Authors. This chapter was originally published under a CC BY-NC 4.0 license, but has now been made available under a CC BY 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBrain cortexen
dc.subjectMitochondriaen
dc.subjectCapillariesen
dc.subjectTensionen
dc.subjectPressureen
dc.subjectVenulesen
dc.subjectSurfaceen
dc.subjectDiseaseen
dc.subjectOxygenen
dc.subjectBrainen
dc.subjectVasculatureen
dc.subjectConfocal microscopeen
dc.titleIn vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissueen
dc.typeConference itemen
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