Position-dependent termination and widespread obligatory frameshifting in Euplotes translation
dc.contributor.author | Lobanov, Alexei V. | |
dc.contributor.author | Heaphy, Stephen M. | |
dc.contributor.author | Turanov, Anton A. | |
dc.contributor.author | Gerashchenko, Maxim V. | |
dc.contributor.author | Pucciarelli, Sandra | |
dc.contributor.author | Devaraj, Raghul R. | |
dc.contributor.author | Xie, Fang | |
dc.contributor.author | Petyuk, Vladislav A. | |
dc.contributor.author | Smith, Richard D. | |
dc.contributor.author | Klobutcher, Lawrence A. | |
dc.contributor.author | Atkins, John F. | |
dc.contributor.author | Miceli, Cristina | |
dc.contributor.author | Hatfield, Dolph L. | |
dc.contributor.author | Baranov, Pavel V. | |
dc.contributor.author | Gladyshev, Vadim N. | |
dc.contributor.funder | European Cooperation in Science and Technology | |
dc.contributor.funder | Ministero dell’Istruzione, dell’Università e della Ricerca | |
dc.contributor.funder | U.S. Department of Energy | |
dc.contributor.funder | Pacific Northwest National Laboratory | |
dc.contributor.funder | Science Foundation Ireland | |
dc.contributor.funder | Wellcome Trust | |
dc.contributor.funder | National Institutes of Health | |
dc.date.accessioned | 2018-07-30T10:30:30Z | |
dc.date.available | 2018-07-30T10:30:30Z | |
dc.date.issued | 2017 | |
dc.description.abstract | The ribosome can change its reading frame during translation in a process known as programmed ribosomal frameshifting. These rare events are supported by complex mRNA signals. However, we found that the ciliates Euplotes crassus and Euplotes focardii exhibit widespread frameshifting at stop codons. 47 different codons preceding stop signals resulted in either +1 or +2 frameshifts, and +1 frameshifting at AAA was the most frequent. The frameshifts showed unusual plasticity and rapid evolution, and had little influence on translation rates. The proximity of a stop codon to the 3' mRNA end, rather than its occurrence or sequence context, appeared to designate termination. Thus, a 'stop codon' is not a sufficient signal for translation termination, and the default function of stop codons in Euplotes is frameshifting, whereas termination is specific to certain mRNA positions and probably requires additional factors. | en |
dc.description.sponsorship | National Institutes of Health (GM061603, GM065402, GM103493); Wellcome Trust (094423); Pacific Northwest National Laboratory/U.S. Department of Energy (Environmental Molecular Science Laboratory); U.S. Department of Energy (DE-AC05-76RLO-1830); Ministero dell’Istruzione, dell’Università e della Ricerca (Programma nazionale di Ricerche in Antartide); European Cooperation in Science and Technology (COST action (BM1102) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Accepted Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural and Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330 | en |
dc.identifier.doi | 10.1038/nsmb.3330 | |
dc.identifier.endpage | 68 | |
dc.identifier.issn | 1545-9993 | |
dc.identifier.issued | 1 | |
dc.identifier.journaltitle | Nature Structural and Molecular Biology | en |
dc.identifier.startpage | 61 | |
dc.identifier.uri | https://hdl.handle.net/10468/6518 | |
dc.identifier.volume | 24 | |
dc.language.iso | en | en |
dc.publisher | Nature Publishing Group | en |
dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IA/1335/IE/Development of computational resources for the analysis of Genome Wide Information on Protein Synthesis (GWIPS)./ | |
dc.relation.uri | https://www.nature.com/articles/nsmb.3330 | |
dc.rights | © 2017, Nature America, Inc., part of Springer Nature. All rights reserved. This is the peer reviewed version of the following article: Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural &Amp; Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330, which has been published in final form at https://doi.org/10.1038/nsmb.3330. | en |
dc.subject | Ciliate | en |
dc.subject | DNA | en |
dc.subject | Slippage | en |
dc.subject | Scale | en |
dc.subject | Yeast | en |
dc.subject | Gene | en |
dc.subject | mRNA | en |
dc.title | Position-dependent termination and widespread obligatory frameshifting in Euplotes translation | en |
dc.type | Article (peer-reviewed) | en |