Position-dependent termination and widespread obligatory frameshifting in Euplotes translation

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dc.contributor.authorLobanov, Alexei V.
dc.contributor.authorHeaphy, Stephen M.
dc.contributor.authorTuranov, Anton A.
dc.contributor.authorGerashchenko, Maxim V.
dc.contributor.authorPucciarelli, Sandra
dc.contributor.authorDevaraj, Raghul R.
dc.contributor.authorXie, Fang
dc.contributor.authorPetyuk, Vladislav A.
dc.contributor.authorSmith, Richard D.
dc.contributor.authorKlobutcher, Lawrence A.
dc.contributor.authorAtkins, John F.
dc.contributor.authorMiceli, Cristina
dc.contributor.authorHatfield, Dolph L.
dc.contributor.authorBaranov, Pavel V.
dc.contributor.authorGladyshev, Vadim N.
dc.contributor.funderEuropean Cooperation in Science and Technology
dc.contributor.funderMinistero dell’Istruzione, dell’Università e della Ricerca
dc.contributor.funderU.S. Department of Energy
dc.contributor.funderPacific Northwest National Laboratory
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderWellcome Trust
dc.contributor.funderNational Institutes of Health
dc.date.accessioned2018-07-30T10:30:30Z
dc.date.available2018-07-30T10:30:30Z
dc.date.issued2017
dc.description.abstractThe ribosome can change its reading frame during translation in a process known as programmed ribosomal frameshifting. These rare events are supported by complex mRNA signals. However, we found that the ciliates Euplotes crassus and Euplotes focardii exhibit widespread frameshifting at stop codons. 47 different codons preceding stop signals resulted in either +1 or +2 frameshifts, and +1 frameshifting at AAA was the most frequent. The frameshifts showed unusual plasticity and rapid evolution, and had little influence on translation rates. The proximity of a stop codon to the 3' mRNA end, rather than its occurrence or sequence context, appeared to designate termination. Thus, a 'stop codon' is not a sufficient signal for translation termination, and the default function of stop codons in Euplotes is frameshifting, whereas termination is specific to certain mRNA positions and probably requires additional factors.en
dc.description.sponsorshipNational Institutes of Health (GM061603, GM065402, GM103493); Wellcome Trust (094423); Pacific Northwest National Laboratory/U.S. Department of Energy (Environmental Molecular Science Laboratory); U.S. Department of Energy (DE-AC05-76RLO-1830); Ministero dell’Istruzione, dell’Università e della Ricerca (Programma nazionale di Ricerche in Antartide); European Cooperation in Science and Technology (COST action (BM1102)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural and Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330en
dc.identifier.doi10.1038/nsmb.3330
dc.identifier.endpage68
dc.identifier.issn1545-9993
dc.identifier.issued1
dc.identifier.journaltitleNature Structural and Molecular Biologyen
dc.identifier.startpage61
dc.identifier.urihttps://hdl.handle.net/10468/6518
dc.identifier.volume24
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IA/1335/IE/Development of computational resources for the analysis of Genome Wide Information on Protein Synthesis (GWIPS)./
dc.relation.urihttps://www.nature.com/articles/nsmb.3330
dc.rights© 2017, Nature America, Inc., part of Springer Nature. All rights reserved. This is the peer reviewed version of the following article: Lobanov, A. V., Heaphy, S. M., Turanov, A. A., Gerashchenko, M. V., Pucciarelli, S., Devaraj, R. R., Xie, F., Petyuk, V. A., Smith, R. D., Klobutcher, L. A., Atkins, J. F., Miceli, C., Hatfield, D. L., Baranov, P. V. and Gladyshev, V. N. (2016) 'Position-dependent termination and widespread obligatory frameshifting in Euplotes translation', Nature Structural &Amp; Molecular Biology, 24, pp. 61–68. doi: 10.1038/nsmb.3330, which has been published in final form at https://doi.org/10.1038/nsmb.3330.en
dc.subjectCiliateen
dc.subjectDNAen
dc.subjectSlippageen
dc.subjectScaleen
dc.subjectYeasten
dc.subjectGeneen
dc.subjectmRNAen
dc.titlePosition-dependent termination and widespread obligatory frameshifting in Euplotes translationen
dc.typeArticle (peer-reviewed)en
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