Development and characterisation of macro-disc and micro-band electrodes for electrochemical sensing applications

Simple item page
dc.availability.bitstreamopenaccess
dc.contributor.advisorGalvin, Paul
dc.contributor.advisorO'Riordan, Alan
dc.contributor.advisorexternalThompson, Michaelen
dc.contributor.authorMadden, Julia
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderElectronic Components and Systems for European Leadershipen
dc.date.accessioned2023-05-17T10:55:27Z
dc.date.available2023-05-17T10:55:27Z
dc.date.issued2022
dc.date.submitted2022
dc.description.abstractThe aim of this PhD thesis was to investigate potential next generation sensor platforms for electrochemical biosensor developments, specifically towards health monitoring applications. With increasing interest in the integration of miniaturised electrodes with minimally invasive and wearable devices, this thesis sought to explore electrodes fabricated using three different technologies for the construction of electrochemical biosensors: Silicon microfabrication, Laser scribing, and dispense printing. The first experimental section aimed to investigate the use of a single ultramicroband electrode fabricated on silicon for mediator-free glucose monitoring in bio-fluid environments. Six ultramicroband electrodes, a counter electrode and reference electrode were fabricated using standard microfabrication methods i.e. lithography and etching techniques. Glucose oxidase was selected as a model enzyme to attach onto a platinum modified gold microband electrode by electropolymerisation with an o-phenylenediamine/ß-cyclodextrin layer. The resulting microband biosensor demonstrated on-chip glucose detection in buffer based media. When applied to foetal bovine serum the sensor displayed a reduced sensitivity. The second experimental section explores the use of laser-scribed graphitic carbon for flexible sensing applications. A facile fabrication method was assessed involving electrodeposition of platinum followed by two casting steps to functionalise electrodes. This study examined the chronoamperometric response of the enzymatic lactate sensor whilst the flexible polyimide substrates were fixed at a curvature (K) of 0.14 mm-1. No noticeable change in signal response was observed in comparison to calibrations obtained with a flat substrate (K=0 mm-1), suggesting potential opportunities for sensor attachment or integration with oral-care products such as mouth swabs and mouth guards. Both laser scribed graphitic carbon and Ag/AgCl modified-laser scribed graphitic carbon were examined as reference electrodes for chronoamperometric lactate measurements. This device was applied for measuring lactate concentrations in artificial saliva and diluted sterile human serum. Finally, this study investigates the potential for a low cost additive printing tool to enable the fabrication of electrochemical sensor devices. To do this, electrodes were designed and printed onto polyimide substrates. Reproducibility between electrode dimensions was assessed using 3D microscopy. Standard electrochemical characterisation techniques were employed to study the reproducibility between electrode electrochemical response. Functionality was also assessed whilst electrodes were fixed were fixed at a curvature (K) of 0.14 mm-1. Finally, a simple casting approach was applied to the dispense printed working electrode to construct a lactate biosensor for a proof of concept electrochemical sensor demonstration.en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMadden, J. 2022. Development and characterisation of macro-disc and micro-band electrodes for electrochemical sensing applications. PhD Thesis, University College Cork.en
dc.identifier.endpage158en
dc.identifier.urihttps://hdl.handle.net/10468/14471
dc.language.isoenen
dc.publisherUniversity College Corken
dc.relation.projectElectronic Components and Systems for European Leadership Grant number (ECSEL 2017-2-783132)
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/EU Joint Programming Initiative::Electronic Components and Systems for European Leadership (ECSEL) Joint Undertaking/18/ECSEL/3499/IE/POSITION-II: A pilot line for the next generation of smart catheters and implants/
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2289/IE/INSIGHT - Irelands Big Data and Analytics Research Centre/
dc.rights© 2022, Julia Madden.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectFlexible sensorsen
dc.subjectMicrobandsen
dc.subjectLactate sensingen
dc.subjectGlucose sensingen
dc.subjectAdditive printingen
dc.subjectElectrochemical biosensoren
dc.subjectLaser scribingen
dc.titleDevelopment and characterisation of macro-disc and micro-band electrodes for electrochemical sensing applicationsen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD - Doctor of Philosophyen
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
MaddenJ_PhD2022.pdf
Size:
8.87 MB
Format:
Adobe Portable Document Format
Description:
Full Text E-thesis
Download
Thumbnail Image
Name:
Submission for Examination form.pdf
Size:
334.82 KB
Format:
Adobe Portable Document Format
Description:
Submission for Examination Form
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
5.2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Theses
Chemistry - Doctoral Theses
College of Science, Engineering and Food Science - Doctoral Theses
Electrical and Electronic Engineering - Doctoral Theses
Tyndall National Institute - Doctoral Theses