A dairy-derived ghrelinergic hydrolysate modulates food intake in vivo

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dc.contributor.authorHowick, Ken
dc.contributor.authorWallace-Fitzsimons, Shauna E.
dc.contributor.authorKandil, Dalia
dc.contributor.authorChruścicka, Barbara
dc.contributor.authorCalis, Mert
dc.contributor.authorMurphy, Eoin
dc.contributor.authorMurray, Brian A.
dc.contributor.authorFernandez, Ayoa
dc.contributor.authorBarry, Kate M.
dc.contributor.authorKelly, Phil M.
dc.contributor.authorRyan, Aoife M.
dc.contributor.authorCryan, John F.
dc.contributor.authorGriffin, Brendan T.
dc.contributor.authorSchellekens, Harriët
dc.contributor.funderEnterprise Irelanden
dc.date.accessioned2019-10-02T04:54:38Z
dc.date.available2019-10-02T04:54:38Z
dc.date.issued2018-09-15
dc.description.abstractRecent times have seen an increasing move towards harnessing the health-promoting benefits of food and dietary constituents while providing scientific evidence to substantiate their claims. In particular, the potential for bioactive protein hydrolysates and peptides to enhance health in conjunction with conventional pharmaceutical therapy is being investigated. Dairy-derived proteins have been shown to contain bioactive peptide sequences with various purported health benefits, with effects ranging from the digestive system to cardiovascular circulation, the immune system and the central nervous system. Interestingly, the ability of dairy proteins to modulate metabolism and appetite has recently been reported. The ghrelin receptor (GHSR-1a) is a G-protein coupled receptor which plays a key role in the regulation of food intake. Pharmacological manipulation of the growth hormone secretagogue receptor-type 1a (GHSR-1a) receptor has therefore received a lot of attention as a strategy to combat disorders of appetite and body weight, including age-related malnutrition and the progressive muscle wasting syndrome known as cachexia. In this study, a milk protein-derivative is shown to increase GHSR-1a-mediated intracellular calcium signalling in a concentration-dependent manner in vitro. Significant increases in calcium mobilisation were also observed in a cultured neuronal cell line heterologously expressing the GHS-R1a. In addition, both additive and synergistic effects were observed following co-exposure of GHSR-1a to both the hydrolysate and ghrelin. Subsequent in vivo studies monitored standard chow intake in healthy male and female Sprague-Dawley rats after dosing with the casein hydrolysate (CasHyd). Furthermore, the provision of gastro-protected oral delivery to the bioactive in vivo may aid in the progression of in vitro efficacy to in vivo functionality. In summary, this study reports a ghrelin-stimulating bioactive peptide mixture (CasHyd) with potent effects in vitro. It also provides novel and valuable translational data supporting the potential role of CasHyd as an appetite-enhancing bioactive. Further mechanistic studies are required in order to confirm efficacy as a ghrelinergic bioactive in susceptible population groups.en
dc.description.sponsorshipEnterprise Ireland (TC20130001)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid2780en
dc.identifier.citationHowick, K., Wallace-Fitzsimons, S., Kandil, D., Chruścicka, B., Calis, M., Murphy, E., Murray, B., Fernandez, A., Barry, K., Kelly, P. and Ryan, A., 2018. A Dairy-Derived Ghrelinergic Hydrolysate Modulates Food Intake In Vivo. International journal of molecular sciences, 19(9), 2780 (21pp). DOI:10.3390/ijms19092780en
dc.identifier.doi10.3390/ijms19092780en
dc.identifier.eissn1422-0067
dc.identifier.endpage21en
dc.identifier.issn1661-6596
dc.identifier.issued9en
dc.identifier.journaltitleInternational Journal of Molecular Sciencesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8672
dc.identifier.volume19en
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.urihttps://www.mdpi.com/1422-0067/19/9/2780
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectGhrelinen
dc.subjectGhrelin receptoren
dc.subjectBioactive peptidesen
dc.subjectDairyen
dc.subjectFood intakeen
dc.subjectAppetiteen
dc.subjectCalcium mobilisationen
dc.subjectCachexiaen
dc.subjectMalnutritionen
dc.titleA dairy-derived ghrelinergic hydrolysate modulates food intake in vivoen
dc.typeArticle (peer-reviewed)en
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