The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation

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dc.contributor.authorSantos-Martínez, María José Ose
dc.contributor.authorInkielewicz-Stȩpniak, Iwona
dc.contributor.authorMedina, Carlos
dc.contributor.authorRahme, Kamil
dc.contributor.authorD'Arcy, Deirdre M.
dc.contributor.authorFox, Daniel J.
dc.contributor.authorHolmes, Justin D.
dc.contributor.authorZhang, Hongzhou
dc.contributor.authorRadomski, Marek Witold
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2016-02-10T17:14:34Z
dc.date.available2016-02-10T17:14:34Z
dc.date.issued2012-01-13
dc.date.updated2013-03-07T20:54:15Z
dc.description.abstractInteractions between blood platelets and nanoparticles have both pharmacological and toxicological significance and may lead to platelet activation and aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for the measurement of platelet microaggregation under flow conditions using a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The aim of the current study was to investigate if QCM-D could be used for the measurement of nanoparticle-platelet interactions. Silica, polystyrene, and gold nanoparticles were tested. The interactions were also studied using light aggregometry and flow cytometry, which measured surface abundance of platelet receptors. Platelet activation was imaged using phase contrast and scanning helium ion microscopy. QCM-D was able to measure nanoparticle-induced platelet microaggregation for all nanoparticles tested at concentrations that were undetectable by light aggregometry and flow cytometry. Microaggregates were measured by changes in frequency and dissipation, and the presence of platelets on the sensor surface was confirmed and imaged by phase contrast and scanning helium ion microscopy.en
dc.description.sponsorshipScience Foundation Ireland (SFI (PI 05/FE1/B862), (RFP/BMT2781), and SFI grant on the BioNanoInteract Ireland project.)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSANTOS-MARTINEZ, M. J., INKIELEWICZ-STEPNIAK, I., MEDINA, C., RAHME, K., D'ARCY, D. M., FOX, D., HOLMES, J. D., ZHANG, H. Z. & RADOMSKI, M. W. 2012. The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation. International Journal of Nanomedicine, 7, 243-255. http://dx.doi.org/10.2147/IJN.S26679en
dc.identifier.doi10.2147/ijn.s26679
dc.identifier.endpage255en
dc.identifier.issn1178-2013
dc.identifier.journaltitleInternational Journal of Nanomedicineen
dc.identifier.startpage243en
dc.identifier.urihttps://hdl.handle.net/10468/2273
dc.identifier.volume7en
dc.language.isoenen
dc.publisherDove Pressen
dc.relation.urihttp://www.dovepress.com/international-journal-of-nanomedicine-journal
dc.rights© 2012 The Authors; publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectPlatelet aggregationen
dc.subjectNanoparticlesen
dc.subjectLight aggregometeren
dc.subjectQuartz crystal microbalance with dissipationen
dc.subjectScanning helium ion microscopyen
dc.subjectGold nanoparticleen
dc.subjectPolystyreneen
dc.subjectSilicon dioxideen
dc.subjectThrombocyte receptoren
dc.subjectQCM-Den
dc.subjectPADGEM proteinen
dc.subjectHeliumen
dc.titleThe use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregationen
dc.typeArticle (peer-reviewed)en
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