The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation
dc.contributor.author | Santos-Martínez, María José Ose | |
dc.contributor.author | Inkielewicz-Stȩpniak, Iwona | |
dc.contributor.author | Medina, Carlos | |
dc.contributor.author | Rahme, Kamil | |
dc.contributor.author | D'Arcy, Deirdre M. | |
dc.contributor.author | Fox, Daniel J. | |
dc.contributor.author | Holmes, Justin D. | |
dc.contributor.author | Zhang, Hongzhou | |
dc.contributor.author | Radomski, Marek Witold | |
dc.contributor.funder | Science Foundation Ireland | en |
dc.date.accessioned | 2016-02-10T17:14:34Z | |
dc.date.available | 2016-02-10T17:14:34Z | |
dc.date.issued | 2012-01-13 | |
dc.date.updated | 2013-03-07T20:54:15Z | |
dc.description.abstract | Interactions between blood platelets and nanoparticles have both pharmacological and toxicological significance and may lead to platelet activation and aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for the measurement of platelet microaggregation under flow conditions using a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The aim of the current study was to investigate if QCM-D could be used for the measurement of nanoparticle-platelet interactions. Silica, polystyrene, and gold nanoparticles were tested. The interactions were also studied using light aggregometry and flow cytometry, which measured surface abundance of platelet receptors. Platelet activation was imaged using phase contrast and scanning helium ion microscopy. QCM-D was able to measure nanoparticle-induced platelet microaggregation for all nanoparticles tested at concentrations that were undetectable by light aggregometry and flow cytometry. Microaggregates were measured by changes in frequency and dissipation, and the presence of platelets on the sensor surface was confirmed and imaged by phase contrast and scanning helium ion microscopy. | en |
dc.description.sponsorship | Science Foundation Ireland (SFI (PI 05/FE1/B862), (RFP/BMT2781), and SFI grant on the BioNanoInteract Ireland project.) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | SANTOS-MARTINEZ, M. J., INKIELEWICZ-STEPNIAK, I., MEDINA, C., RAHME, K., D'ARCY, D. M., FOX, D., HOLMES, J. D., ZHANG, H. Z. & RADOMSKI, M. W. 2012. The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation. International Journal of Nanomedicine, 7, 243-255. http://dx.doi.org/10.2147/IJN.S26679 | en |
dc.identifier.doi | 10.2147/ijn.s26679 | |
dc.identifier.endpage | 255 | en |
dc.identifier.issn | 1178-2013 | |
dc.identifier.journaltitle | International Journal of Nanomedicine | en |
dc.identifier.startpage | 243 | en |
dc.identifier.uri | https://hdl.handle.net/10468/2273 | |
dc.identifier.volume | 7 | en |
dc.language.iso | en | en |
dc.publisher | Dove Press | en |
dc.relation.uri | http://www.dovepress.com/international-journal-of-nanomedicine-journal | |
dc.rights | © 2012 The Authors; publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/3.0/ | en |
dc.subject | Platelet aggregation | en |
dc.subject | Nanoparticles | en |
dc.subject | Light aggregometer | en |
dc.subject | Quartz crystal microbalance with dissipation | en |
dc.subject | Scanning helium ion microscopy | en |
dc.subject | Gold nanoparticle | en |
dc.subject | Polystyrene | en |
dc.subject | Silicon dioxide | en |
dc.subject | Thrombocyte receptor | en |
dc.subject | QCM-D | en |
dc.subject | PADGEM protein | en |
dc.subject | Helium | en |
dc.title | The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation | en |
dc.type | Article (peer-reviewed) | en |