Engineered reversal of function in glycolytic yeast promoters

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dc.contributor.authorRajkumar, Arun S.
dc.contributor.authorĂ–zdemir, Emre
dc.contributor.authorLis, Alicia V.
dc.contributor.authorSchneider, Konstantin
dc.contributor.authorQin, Jiufu
dc.contributor.authorJensen, Michael K.
dc.contributor.authorKeasling, Jay D.
dc.contributor.funderNovo Nordisk Fondenen
dc.contributor.funderHorizon 2020en
dc.date.accessioned2019-09-17T13:56:38Z
dc.date.available2019-09-17T13:56:38Z
dc.date.issued2019-05-03
dc.description.abstractPromoters are key components of cell factory design, allowing precise expression of genes in a heterologous pathway. Several commonly used promoters in yeast cell factories belong to glycolytic genes, highly expressed in actively growing yeast when glucose is used as a carbon source. However, their expression can be suboptimal when alternate carbon sources are used, or if there is a need to decouple growth from production. Hence, there is a need for alternate promoters for different carbon sources and production schemes. In this work, we demonstrate a reversal of regulatory function in two glycolytic yeast promoters by replacing glycolytic regulatory elements with ones induced by the diauxic shift. We observe a shift in induction from glucose-rich to glucose-poor medium without loss of regulatory activity, and strong ethanol induction. Applications of these promoters were validated for expression of the vanillin biosynthetic pathway, reaching production of vanillin comparable to pathway designs using strong constitutive promoters.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRajkumar, A. S., Ă–zdemir, E., Lis, A. V., Schneider, K., Qin, J., Jensen, M. K. and Keasling, J. D. (2019) 'Engineered Reversal of Function in Glycolytic Yeast Promoters', ACS Synthetic Biology, 8(6), pp. 1462-1468. (6pp.) DOI: 10.1021/acssynbio.9b00027en
dc.identifier.doi10.1021/acssynbio.9b00027en
dc.identifier.eissn2161-5063
dc.identifier.endpage1468en
dc.identifier.issued6en
dc.identifier.journaltitleACS Synthetic Biologyen
dc.identifier.startpage1462en
dc.identifier.urihttps://hdl.handle.net/10468/8545
dc.identifier.volume8en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/613771/EU/Development of 2nd Generation Biorefineries – Production of Dicarboxylic Acids and Bio-based Polymers Derived Thereof/BIOREFINE-2Gen
dc.relation.urihttps://pubs.acs.org/doi/10.1021/acssynbio.9b00027
dc.rights©2019 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.en
dc.rights.urihttps://pubs.acs.org/page/policy/authorchoice_termsofuse.htmlen
dc.subjectTranscriptomeen
dc.subjectSynthetic promoteren
dc.subjectGlycolysisen
dc.subjectGluconeogenesisen
dc.subjectVanillinen
dc.titleEngineered reversal of function in glycolytic yeast promotersen
dc.typeArticle (peer-reviewed)en
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