The exploration of potato-associated bacteria in the Central Andean Highlands and their application in integrated crop management systems

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dc.check.date10000-01-01
dc.check.embargoformatBoth hard copy thesis and e-thesisen
dc.check.entireThesisEntire Thesis Restricted
dc.check.infoIndefiniteen
dc.check.opt-outNot applicableen
dc.check.reasonThis thesis contains third party copyrighted materials for which permission was not given for online useen
dc.contributor.advisorDoyle Prestwich, Barbaraen
dc.contributor.authorVelivelli, Siva L. S.
dc.contributor.funderSeventh Framework Programmeen
dc.date.accessioned2016-12-08T09:53:29Z
dc.date.issued2014
dc.date.submitted2014
dc.description.abstractPotato is the most important food crop after wheat and rice. A changing climate, coupled with a heightened consumer awareness of how food is produced and legislative changes governing the usage of agrochemicals, means that alternative more integrated and sustainable approaches are needed for crop management practices. Bioprospecting in the Central Andean Highlands resulted in the isolation and in vitro screening of 600 bacterial isolates. The best performing isolates, under in vitro conditions, were field trialled in their home countries. Six of the isolates, Pseudomonas sp. R41805 (Bolivia), Pseudomonas palleroniana R43631 (Peru), Bacillus sp. R47065, R47131, Paenibacillus sp. B3a R49541, and Bacillus simplex M3-4 R49538 (Ecuador), showed significant increase in the yield of potato. Using – omic technologies (i.e. volatilomic, transcriptomic, proteomic and metabolomic), the influence of microbial isolates on plant defence responses was determined. Volatile organic compounds of bacterial isolates were identified using GC/MS. RT-qPCR analysis revealed the significant expression of Ethylene Response Factor 3 (ERF3) and the results of this study suggest that the dual inoculation of potato with Pseudomonas sp. R41805 and Rhizophagus irregularis MUCL 41833 may play a part in the activation of plant defence system via ERF3. The proteomic analysis by 2-DE study has shown that priming by Pseudomonas sp. R41805 can induce the expression of proteins related to photosynthesis and protein folding in in vitro potato plantlets. The metabolomics study has shown that the total glycoalkaloid (TGA) content of greenhouse-grown potato tubers following inoculation with Pseudomonas sp. R41805 did not exceed the acceptable safety limit (200 mg kg-1 FW). As a result of this study, a number of bacteria have been identified with commercial potential that may offer sustainable alternatives in both Andean and European agricultural settings.en
dc.description.sponsorshipSeventh Framework Programme (EU-FP7 grant agreement No 227522)en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationVelivelli, S. L. S. 2014. The exploration of potato-associated bacteria in the Central Andean Highlands and their application in integrated crop management systems. PhD Thesis, University College Cork.en
dc.identifier.endpage208en
dc.identifier.urihttps://hdl.handle.net/10468/3357
dc.languageEnglishen
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2014, Siva L.S. Velivelli.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectRhizoctonia solanien
dc.subjectPhytophthora infestansen
dc.subjectPotatoen
dc.subjectArbuscular mycorrhizal fungien
dc.subjectPlant growth-promoting rhizobacteriaen
dc.subjectVolatile organic compoundsen
dc.subjectGas chromatography/mass spectrometryen
dc.subjectEthylene response factor 3en
dc.subjectSustainable agricultureen
dc.subjectBiological controlen
dc.subjectTwo-dimensional gel electrophoresisen
dc.subjectDefence gene expressionen
dc.thesis.opt-outfalse
dc.titleThe exploration of potato-associated bacteria in the Central Andean Highlands and their application in integrated crop management systemsen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Science)en
ucc.workflow.supervisorb.doyle@ucc.ie
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