Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens

dc.contributor.authorRiza, Nabeel A.
dc.contributor.authorSheikh, Mumtaz A.
dc.contributor.authorWebb-Wood, Grady
dc.contributor.authorKik, Pieter G.
dc.date.accessioned2020-05-25T14:45:54Z
dc.date.available2020-05-25T14:45:54Z
dc.date.issued2008-06-01
dc.description.abstractThree-dimensional (3-D) imaging is demonstrated using an electronically controlled liquid crystal (LC) optical lens to accomplish a no-moving-parts depth-section scanning in a modified commercial 3-D confocal microscope. Specifically, 3-D views of a standard CDC blood vessel (enclosed in a glass slide) have been obtained using the modified confocal microscope operating at the red 633-nm laser wavelength. The image sizes over a 25-μm axial scan depth were 50×50 μm and 80×80 μm, using 60× and 20× micro-objectives, respectively. The transverse motion step was 0.1 μm for the 60× data and 0.2 μm for the 20× data. As a first-step comparison, image processing of the standard and LC electronic-lens microscope images indicates correlation values between 0.81 and 0.91. The proposed microscopy system within aberration limits has the potential to eliminate the mechanical forces due to sample or objective motion that can distort the original sample structure and lead to imaging errors.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid063201en
dc.identifier.citationRiza, N. A., Sheikh, M. A., Webb-Wood, G. and Kik, P. K. (2008) ‘Demonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lens’, Optical Engineering, 47(6), 063201 (9 pp). doi: 10.1117/1.2944135en
dc.identifier.doi10.1117/1.2944135en
dc.identifier.endpage9en
dc.identifier.issn0091-3286
dc.identifier.issn1560-2303
dc.identifier.issued8en
dc.identifier.journaltitleOptical Engineeringen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/10043
dc.identifier.volume47en
dc.language.isoenen
dc.publisherSociety of Photo-optical Instrumentation Engineers (SPIE)en
dc.rights© 2008 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.en
dc.subjectLiquid crystalen
dc.subjectOptical imagingen
dc.subjectMicroscopyen
dc.subjectMicroscopesen
dc.subjectConfocal microscopyen
dc.subject3D image processingen
dc.subjectImaging systemsen
dc.subjectMonochromatic aberrationsen
dc.subjectArteriesen
dc.titleDemonstration of three-dimensional optical imaging using a confocal microscope based on a liquid-crystal electronic lensen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Demonstration of three-dimensional optical.pdf
Size:
1.2 MB
Format:
Adobe Portable Document Format
Description:
Published version
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description: