dc.contributor.author	Schossleitner, Klaudia
dc.contributor.author	O'Mahony, Conor
dc.contributor.author	Brandstätter, Stefan
dc.contributor.author	Haslinger, Michael J.
dc.contributor.author	Demuth, Sabrina
dc.contributor.author	Fechtig, Daniel
dc.contributor.author	Petzelbauer, Peter
dc.contributor.funder	Österreichische Forschungsförderungsgesellschaft	en
dc.date.accessioned	2019-07-16T15:44:46Z
dc.date.available	2019-07-16T15:44:46Z
dc.date.issued	2018-11-19
dc.description.abstract	Microneedles are promising devices for transdermal delivery and diagnostic applications, due to their minimally invasive and painless nature of application. However, so far, applications are limited to small scale research projects. Material selection and production for larger projects remain a challenge. In vitro testing using human cell culture could bridge the gap between cost effective screening of suitable materials and concerns for safety and ethics. In this study, materials were tested for effects on viability and morphology of human endothelial cells and keratinocytes. In addition, materials were assessed for their potential to influence cellular differentiation and barrier formation. Elution-based testing of inflammatory markers revealed no negative effects in all applied tests, whereas the assessment of differentiation markers on cells in direct contact with the material showed differences and allowed the selection of candidate materials for future medical device applications. This study illustrates that elution-based biocompatibility testing can paint an incomplete picture. Advanced staining techniques and cell types specific for the application of the medical device improve material selection to reduce and replace animal testing at an early stage in the development process. © 2018 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 505–512, 2019.	en
dc.description.sponsorship	Österreichische Forschungsförderungsgesellschaft (Project 853482 Microneedle)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Schossleitner, K., O'Mahony, C., Brandstätter, S., Haslinger, M.J., Demuth, S., Fechtig, D. and Petzelbauer, P., 2019. Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells. Journal of Biomedical Materials Research Part A, 107(3), 505-512. DOI:10.1002/jbm.a.36565	en
dc.identifier.doi	10.1002/jbm.a.36565	en
dc.identifier.eissn	1097-4636
dc.identifier.endpage	512	en
dc.identifier.issn	0021-9304
dc.identifier.issued	3	en
dc.identifier.journaltitle	Journal of Biomedical Materials Research Part A	en
dc.identifier.startpage	505	en
dc.identifier.uri	https://hdl.handle.net/10468/8177
dc.identifier.volume	107	en
dc.language.iso	en	en
dc.publisher	John Wiley and Sons Inc.	en
dc.relation.uri	https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36565
dc.rights	© 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	en
dc.subject	Microneedle	en
dc.subject	Biocompatibility	en
dc.subject	Keratinocytes	en
dc.subject	Replacement	en
dc.subject	Cyclic olefin polymer	en
dc.title	Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells	en
dc.type	Article (peer-reviewed)	en

Differences in biocompatibility of microneedles from cyclic olefin polymers with human endothelial and epithelial skin cells

Files

Original bundle

License bundle

Collections