Molecular signature of Pseudomonas aeruginosa with simultaneous nanomolar detection of quorum sensing signaling molecules at a Boron-Doped diamond electrode

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dc.contributor.authorBuzid, Alyah
dc.contributor.authorShang, Fengjun
dc.contributor.authorReen, F. Jerry
dc.contributor.authorÓ Muimhneacháin, Eoin
dc.contributor.authorClarke, Sarah L.
dc.contributor.authorZhou, Lin
dc.contributor.authorLuong, John H. T.
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorMcGlacken, Gerard P.
dc.contributor.authorGlennon, Jeremy D.
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderEnterprise Ireland
dc.contributor.funderDepartment of Agriculture, Food and the Marine
dc.contributor.funderIrish Research Council
dc.contributor.funderEuropean Commission
dc.contributor.funderMarine Institute
dc.contributor.funderTeagasc
dc.contributor.funderHealth Research Board
dc.contributor.funderIrish Thoracic Society
dc.contributor.funderUniversity College Cork
dc.date.accessioned2017-06-22T13:55:53Z
dc.date.available2017-06-22T13:55:53Z
dc.date.issued2016-07-18
dc.description.abstractElectroanalysis was performed using a boron-doped diamond (BDD) electrode for the simultaneous detection of 2-heptyl-3-hydroxy-4-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ) and pyocyanin (PYO). PQS and its precursor HHQ are two important signal molecules produced by Pseudomonas aeruginosa, while PYO is a redox active toxin involved in virulence and pathogenesis. This Gram-negative and opportunistic human pathogen is associated with a hospital-acquired infection particularly in patients with compromised immunity and is the primary cause of morbidity and mortality in cystic fibrosis (CF) patients. Early detection is crucial in the clinical management of this pathogen, with established infections entering a biofilm lifestyle that is refractory to conventional antibiotic therapies. Herein, a detection procedure was optimized and proven for the simultaneous detection of PYO, HHQ and PQS in standard mixtures, biological samples, and P. aeruginosa spiked CF sputum samples with remarkable sensitivity, down to nanomolar levels. Differential pulse voltammetry (DPV) scans were also applicable for monitoring the production of PYO, HHQ and PQS in P. aeruginosa PA14 over 8 h of cultivation. The simultaneous detection of these three compounds represents a molecular signature specific to this pathogen.en
dc.description.sponsorshipScience Foundation Ireland/Enterprise Ireland (Technology Innovation Development Award (TIDA) (SFI/12/TIDA/B2405). Science Foundation Ireland (SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438; SFI/12/IP/1315; SFI/09/RFP/CHS2353; SSPC2 12/RC/2275; 08/SRC/B1412); Department of Agriculture and Food (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council (Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647); GOIPG/2013/336); Health Research Board/Irish Thoracic Society (MRCG-2014-6); Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013); University College Cork (Strategic Research Fund Ph.D. Studentship)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid30001
dc.identifier.citationBuzid, A., Shang, F., Reen, F. J., Muimhneacháin, E. Ó., Clarke, S. L., Zhou, L., Luong, J. H. T., O’Gara, F., McGlacken, G. P. and Glennon, J. D. (2016) 'Molecular Signature of Pseudomonas aeruginosa with Simultaneous Nanomolar Detection of Quorum Sensing Signaling Molecules at a Boron-Doped Diamond Electrode', Scientific Reports, 6, 30001 (9pp). doi: 10.1038/srep30001en
dc.identifier.doi10.1038/srep30001
dc.identifier.endpage9
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4168
dc.identifier.volume6
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATION
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE
dc.relation.urihttps://www.nature.com/articles/srep30001
dc.rights© 2016, Buzid et al. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectDiagnostic markersen
dc.subjectMedical and clinical diagnosticsen
dc.titleMolecular signature of Pseudomonas aeruginosa with simultaneous nanomolar detection of quorum sensing signaling molecules at a Boron-Doped diamond electrodeen
dc.typeArticle (peer-reviewed)en
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