A non-classical LysR-type transcriptional regulator PA2206 is required for an effective oxidative stress response in Pseudomonas aeruginosa

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dc.contributor.authorReen, F. Jerry
dc.contributor.authorHaynes, Jill M.
dc.contributor.authorMooij, Marlies J.
dc.contributor.authorO'Gara, Fergal
dc.contributor.funderEuropean Commissionen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderDepartment of Agriculture and Food, Irelanden
dc.contributor.funderIrish Research Council for Science Engineering and Technologyen
dc.contributor.funderHealth Research Boarden
dc.contributor.funderEnvironmental Protection Agencyen
dc.contributor.funderMarine Instituteen
dc.contributor.funderHigher Education Authorityen
dc.contributor.funderHealth Service Executive, Irelanden
dc.date.accessioned2016-02-17T11:46:20Z
dc.date.available2016-02-17T11:46:20Z
dc.date.issued2013
dc.description.abstractLysR-type transcriptional regulators (LTTRs) are emerging as key circuit components in regulating microbial stress responses and are implicated in modulating oxidative stress in the human opportunistic pathogen Pseudomonas aeruginosa. The oxidative stress response encapsulates several strategies to overcome the deleterious effects of reactive oxygen species. However, many of the regulatory components and associated molecular mechanisms underpinning this key adaptive response remain to be characterised. Comparative analysis of publically available transcriptomic datasets led to the identification of a novel LTTR, PA2206, whose expression was altered in response to a range of host signals in addition to oxidative stress. PA2206 was found to be required for tolerance to H2O2 in vitro and lethality in vivo in the Zebrafish embryo model of infection. Transcriptomic analysis in the presence of H2O2 showed that PA2206 altered the expression of 58 genes, including a large repertoire of oxidative stress and iron responsive genes, independent of the master regulator of oxidative stress, OxyR. Contrary to the classic mechanism of LysR regulation, PA2206 did not autoregulate its own expression and did not influence expression of adjacent or divergently transcribed genes. The PA2214-15 operon was identified as a direct target of PA2206 with truncated promoter fragments revealing binding to the 5'-ATTGCCTGGGGTTAT-3' LysR box adjacent to the predicted -35 region. PA2206 also interacted with the pvdS promoter suggesting a global dimension to the PA2206 regulon, and suggests PA2206 is an important regulatory component of P. aeruginosa adaptation during oxidative stress.en
dc.description.sponsorshipEuropean Commission (MTKD-CT-2006-042062, O36314, FP7-PEOPLE-2009-RG, EU 256596); Science Foundation Ireland (SFI 04/BR/B0597, 07/IN.1/B948, 08/RFP/GEN1295, 08/RFP/GEN1319, 09/RFP/BMT2350); Department of Agriculture and Food (DAF RSF 06 321, DAF RSF 06 377, FIRM 08/RDC/629); Irish Research Council for Science, Engineering and Technology (05/EDIV/FP107, PD/2011/2414); Health Research Board (RP/2006/271, RP/2007/290, HRA/2009/146); Environmental Protection Agency (EPA2006-PhD-S-21, EPA2008-PhD-S-2); Marine Institute (C2CRA 2007/082); Higher Education Authority of Ireland (PRTLI3); Health Service Executive (HSE) surveillance funden
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide54479
dc.identifier.citationReen FJ, Haynes JM, Mooij MJ, O'Gara F (2013) A Non-Classical LysR-Type Transcriptional Regulator PA2206 Is Required for an Effective Oxidative Stress Response in Pseudomonas aeruginosa. PLoS ONE 8(1): e54479. doi:10.1371/journal.pone.0054479en
dc.identifier.doi10.1371/journal.pone.0054479
dc.identifier.issn1932-6203
dc.identifier.issued1en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2395
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2013 Reen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCystic fibrosis patientsen
dc.subjectEscherichia colien
dc.subjectHydrogen peroxideen
dc.subjectSalmonella typhimuriumen
dc.subjectSinorhizobium melilotien
dc.subjectOpportunistic pathogenen
dc.subjectHuman neutrophilsen
dc.subjectGene expressionen
dc.subjectFull virulenceen
dc.subjectOxyRen
dc.titleA non-classical LysR-type transcriptional regulator PA2206 is required for an effective oxidative stress response in Pseudomonas aeruginosaen
dc.typeArticle (peer-reviewed)en
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