Development of a click beetle luciferase reporter system for enhanced bioluminescence imaging of Listeria monocytogenes: analysis in cell culture and murine infection models

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dc.contributor.authorUr Rahman, Sadeeq
dc.contributor.authorStanton, Michael
dc.contributor.authorCasey, Pat G.
dc.contributor.authorSpagnuolo, Angela
dc.contributor.authorBensi, Giuliano
dc.contributor.authorHill, Colin
dc.contributor.authorFrancis, Kevin P.
dc.contributor.authorTangney, Mark
dc.contributor.authorGahan, Cormac G.
dc.contributor.funderSeventh Framework Programme
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2017-12-08T13:33:47Z
dc.date.available2017-12-08T13:33:47Z
dc.date.issued2017-09-26
dc.description.abstractListeria monocytogenes is a Gram-positive facultative intracellular pathogen that is widely used as a model organism for the analysis of infection biology. In this context, there is a current need to develop improved reporters for enhanced bioluminescence imaging (BLI) of the pathogen in infection models. We have developed a click beetle red luciferase (CBR-luc) based vector (pPL2CBRopt) expressing codon optimized CBR-luc under the control of a highly expressed Listerial promoter (PHELP) for L. monocytogenes and have compared this to a lux-based system expressing bacterial luciferase for BLI of the pathogen using in vitro growth experiments and in vivo models. The CBR-luc plasmid stably integrates into the L. monocytogenes chromosome and can be used to label field isolates and laboratory strains of the pathogen. Growth experiments revealed that CBR-luc labeled L. monocytogenes emits a bright signal in exponential phase that is maintained during stationary phase. In contrast, lux-labeled bacteria produced a light signal that peaked during exponential phase and was significantly reduced during stationary phase. Light from CBR-luc labeled bacteria was more efficient than the signal from lux-labeled bacteria in penetrating an artificial tissue depth assay system. A cell invasion assay using C2Bbe1 cells and a systemic murine infection model revealed that CBR-luc is suited to BLI approaches and demonstrated enhanced sensitivity relative to lux in the context of Listeria infection models. Overall, we demonstrate that this novel CBR reporter system provides efficient, red-shifted light production relative to lux and may have significant applications in the analysis of L. monocytogenes pathogenesisen
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1797
dc.identifier.citationUr Rahman, S., Stanton, M., Casey, P. G., Spagnuolo, A., Bensi, G., Hill, C., Francis, K. P., Tangney, M. and Gahan, C. G. M. (2017) Development of a click beetle luciferase reporter system for enhanced bioluminescence imaging of Listeria monocytogenes: analysis in cell culture and murine infection models', Frontiers in Microbiology, 8,1797 (12pp). doi: 10.3389/fmicb.2017.01797en
dc.identifier.doi10.3389/fmicb.2017.01797
dc.identifier.endpage12
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/5151
dc.identifier.volume8
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/612219/EU/Vaccines and Imaging Partnership/VIP
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2017.01797/full
dc.rights© 2017, Ur Rahman, Stanton, Casey, Spagnuolo, Bensi, Hill, Francis, Tangney and Gahan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectListeria monocytogenesen
dc.subjectClick beetle luciferaseen
dc.subjectBioluminescenceen
dc.subjectIn-vivo imagingen
dc.subjectPathogenen
dc.subjectVirulenceen
dc.subjectClinicalen
dc.titleDevelopment of a click beetle luciferase reporter system for enhanced bioluminescence imaging of Listeria monocytogenes: analysis in cell culture and murine infection modelsen
dc.typeArticle (peer-reviewed)en
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