Cardioprotective and anti-arrhythmic effects of magnesium pretreatment against ischaemia/reperfusion injury in isoprenaline-induced hypertrophic rat heart

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dc.contributor.authorAmoni, Matthew
dc.contributor.authorKelly-Laubscher, Roisin
dc.contributor.authorPetersen, Morea
dc.contributor.authorGwanyanya, Asfree
dc.contributor.funderUniversity of Cape Townen
dc.contributor.funderNational Research Foundationen
dc.date.accessioned2022-01-28T11:08:12Z
dc.date.available2022-01-28T11:08:12Z
dc.date.issued2015-12-22
dc.date.updated2022-01-28T10:58:09Z
dc.description.abstractThe effects of magnesium (Mg2+) on ischaemic complications of pathological cardiac hypertrophy are unclear. In this study, we investigated effects of Mg2+ pretreatment on ischaemia/reperfusion (I/R) injury in isoprenaline (ISO)-induced hypertrophic hearts. Wistar rats were treated for 7 days with different combinations of ISO (1.25 mg/kg) subcutaneously, MgSO4 (270 mg/kg) intraperitoneally, or vehicle (saline). On the eighth day, hearts were either subjected to regional I/R during Langendorff perfusion or histologically stained with haematoxylin and eosin and Masson’s trichrome. Haemodynamic and electrocardiographic parameters were recorded using the PowerLab data-acquisition system. Infarcts were identified by triphenyltetrazolium chloride staining. Plasma Mg2+ was measured using photometric assays. Mg2+ pretreatment significantly decreased I/R-induced infarct size (p = 0.001) and the overall arrhythmia score (p < 0.001) of I/R-induced ventricular ectopics, ventricular tachycardia, and ventricular fibrillation in hypertrophic hearts, but not non-hypertrophied hearts. Mg2+ also improved post-I/R left ventricular developed pressure in hypertrophic hearts. However, Mg2+ did not reverse the ISO-induced myocyte thickening and interstitial fibrosis or increases in heart weight. Plasma Mg2+ was not different among treatment groups. These results suggest that Mg2+ pretreatment may protect against I/R-induced injury and malignant arrhythmias in hypertrophic hearts, possibly via mechanisms unrelated to long-lasting changes in plasma Mg2+ or prevention of structural changes such as fibrosis.en
dc.description.sponsorshipUniversity of Cape Town (UCT Clinical Scholar Programme VC Strategic Award and National Research Foundation (NRF) of South Africa Grant; UCT URC/Carnegie Research Development Grant; UCT Faculty of Health Sciences, and NRF (Grant No. 85768); UCT URC/Carnegie Research Development Grant, NRF (Grant No. 91514)); ADInstruments (Australia) Grant.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAmoni, M., Kelly-Laubscher, R., Petersen, M. and Gwanyanya, A. (2017) 'Cardioprotective and Anti-arrhythmic Effects of Magnesium Pretreatment Against Ischaemia/Reperfusion Injury in Isoprenaline-Induced Hypertrophic Rat Heart', Cardiovascular Toxicology, 17, pp. 49-57. doi: 10.1007/s12012-015-9355-6en
dc.identifier.doi10.1007/s12012-015-9355-6en
dc.identifier.endpage57en
dc.identifier.issn1559-0259
dc.identifier.journaltitleCardiovascular Toxicologyen
dc.identifier.startpage49en
dc.identifier.urihttps://hdl.handle.net/10468/12502
dc.identifier.volume17en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urihttps://link.springer.com/article/10.1007/s12012-015-9355-6
dc.rights© Springer Science+Business Media New York 2015. This version of the article has been accepted for publication, after peer review and is subject to Springer Natures Accepted Manuscript terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://link.springer.com/article/10.1007/s12012-015-9355-6en
dc.rights.urihttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsen
dc.subjectArrhythmiaen
dc.subjectCardiacen
dc.subjectHypertrophyen
dc.subjectIschaemia/reperfusionen
dc.subjectIsoprenalineen
dc.subjectMagnesiumen
dc.titleCardioprotective and anti-arrhythmic effects of magnesium pretreatment against ischaemia/reperfusion injury in isoprenaline-induced hypertrophic rat hearten
dc.typeArticle (peer-reviewed)en
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