Profiling phytochemical and nutritional components of potato

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dc.contributor.advisorO'Brien, Nora M.en
dc.contributor.advisorReilly, Kimen
dc.contributor.advisorGaffney, Michaelen
dc.contributor.authorValcarcel Barroso, Jesus
dc.contributor.funderTeagascen
dc.date.accessioned2015-08-12T10:41:12Z
dc.date.available2015-08-12T10:41:12Z
dc.date.issued2014
dc.date.submitted2014
dc.description.abstractPotatoes (Solanum Tuberosum L.) contain secondary metabolites that may have an impact on human health. The aim of this study was to assess the levels of some of these compounds in a wide range of varieties, including rare, heritage and commercial cultivars. Vitamin C, total carotenoids, phenolics, flavonoids, antioxidant activity and glycoalkaloids were determined, using spectroscopy and chromatography, in the skin and flesh of tubers grown in field trials. Transcript levels of key synthetic enzymes were assessed by qPCR. Accumulation of selected metabolites was higher in the skin than in the flesh of tubers, except ascorbate, which was undetected in the skin. Differences were on average 2.5 to 3-fold for carotenoids, 6-fold for phenolics, 15 to 16-fold for flavonoids, 21-fold for glycoalkaloids and 9 to 10-fold for antioxidant activity. Higher contents of carotenoids were associated with yellow skin or flesh, and higher values of phenolics, flavonoids and antioxidant activity with blue flesh. Variety ‘Burren’ had maxima values of carotenoids in skin and flesh, variety ‘Nicola’ of ascorbate, variety ‘Congo’ of phenolics, flavonoids and antioxidant activity in both tissues, except antioxidant activity in the skin, which was higher in ‘Edzell Blue’. Varieties ‘May Queen’ and ‘International Kidney’ had highest glycoalkaloid content in skin and flesh respectively. The effect of the environment was diverse: year of cultivation was significant for all metabolites, but site of cultivation was not for carotenoids and glycoalkaloids. Levels of expression of phenylalanine ammonia-lyase and chalcone synthase were higher in varieties accumulating high contents of phenolic compounds. However, levels of expression of phytoene synthase and L-galactono-1,4-lactone dehydrogenase were not different between varieties showing contrasting levels of carotenoids and ascorbate respectively. This work will help identify varieties that could be marketed as healthier and the most suitable varieties for extraction of high-value metabolites such as glycoalkaloids.en
dc.description.sponsorshipTeagasc (Walsh Fellowship Program)en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationValcarcel, J. 2014. Profiling phytochemical and nutritional components of potato. PhD Thesis, University College Cork.en
dc.identifier.endpage152
dc.identifier.urihttps://hdl.handle.net/10468/1891
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2014, Jesus Valcarcel.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectSolanum tuberosumen
dc.subjectPCRen
dc.subjectPotatoen
dc.subjectPhytochemicalen
dc.subjectAntioxidanten
dc.subjectVitaminsen
dc.subjectAscorbic aciden
dc.subjectCarotenoidsen
dc.subjectPhenolic compoundsen
dc.subjectFlavonoidsen
dc.subjectGlycoalkaloidsen
dc.subjectGene expressionen
dc.thesis.opt-outfalse
dc.titleProfiling phytochemical and nutritional components of potatoen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Food Science and Technology)en
ucc.workflow.supervisornob@ucc.ie
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