Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content

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dc.contributor.authorOliveira Jr., Getulio P.
dc.contributor.authorPorto, William F.
dc.contributor.authorPalu, Cintia C.
dc.contributor.authorPereira, Lydyane M.
dc.contributor.authorPetriz, Bernardo
dc.contributor.authorAlmeida, Jeeser A.
dc.contributor.authorViana, Juliane
dc.contributor.authorFilho, Nezio N. A.
dc.contributor.authorFranco, Octavio L.
dc.contributor.authorPereira, Rinaldo W.
dc.contributor.funderIrish Research Council
dc.contributor.funderUniversidade Católica de Brasília
dc.contributor.funderFundação de Apoio à Pesquisa do Distrito Federal
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológico
dc.contributor.funderMinistério da Educação
dc.date.accessioned2018-09-24T12:36:55Z
dc.date.available2018-09-24T12:36:55Z
dc.date.issued2018
dc.description.abstractPhysical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other organs communicate to translate exercise into health. Low, moderate and high intensity treadmill protocols were applied to rat groups, aiming to investigate the impact of exercise on serum EVs and their associated small RNA molecules. Transmission electron microscopy, resistive pulse sensing, and western blotting were used to investigate EVs morphology, size distribution, concentration and EVs marker proteins. Small RNA libraries from EVs RNA were sequenced. Exercise did not change EVs size, while increased EVs concentration. Twelve miRNAs were found differentially expressed after exercise: rno-miR-128-3p, 1033p, 330-5p, 148a-3p, 191a-5p, 10b-5p, 93-5p, 25-3p, 142-5p, 3068-3p, 142-3p, and 410-3p. No piRNA was found differentially expressed, and one tRNA, trna8336, was found down-regulated after exercise. The differentially expressed miRNAs were predicted to target genes involved in the MAPK pathway. A single bout of exercise impacts EVs and their small RNA load, reinforcing the need for a more detailed investigation into EVs and their load as mediators of health-promoting exercise.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid532
dc.identifier.citationOliveira, G. P., Porto, W. F., Palu, C. C., Pereira, L. M., Petriz, B., Almeida, J. A., Viana, J., Filho, N. N. A., Franco, O. L. and Pereira, R. W. (2018) 'Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content', Frontiers in Physiology, 9, 532 (11pp). doi: 10.3389/fphys.2018.00532en
dc.identifier.doi10.3389/fphys.2018.00532
dc.identifier.endpage11
dc.identifier.issn1664-042X
dc.identifier.journaltitleFrontiers in Physiologyen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6868
dc.identifier.volume9
dc.language.isoenen
dc.publisherFrontiers Media SAen
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fphys.2018.00532/full
dc.rights© 2018, Oliveira, Porto, Palu, Pereira, Petriz, Almeida, Viana, Filho, Franco and Pereira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectExtracellular vesiclesen
dc.subjectSmall RNAen
dc.subjectAerobic exerciseen
dc.subjectNextgeneen
dc.subjectEdgeren
dc.titleEffects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs contenten
dc.typeArticle (peer-reviewed)en
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