RNA-sequencing analysis of umbilical cord plasma microRNAs from healthy newborns

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dc.contributor.authorBrennan, Gary P.
dc.contributor.authorVitsios, Dimitrios M.
dc.contributor.authorCasey, Sophie
dc.contributor.authorLooney, Ann-Marie
dc.contributor.authorHallberg, Boubou
dc.contributor.authorHenshall, David C.
dc.contributor.authorBoylan, Geraldine B.
dc.contributor.authorMurray, Deirdre M.
dc.contributor.authorMooney, Catherine
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Regional Development Funden
dc.contributor.funderRoyal College of Surgeons in Irelanden
dc.contributor.funderEpilepsy Irelanden
dc.contributor.funderIrish Research Councilen
dc.contributor.funderEuropean Molecular Biology Laboratoryen
dc.contributor.funderMedical Research Charities Groupen
dc.contributor.funderFutureNeuroen
dc.contributor.funderHealth Research Boarden
dc.date.accessioned2019-06-24T13:40:01Z
dc.date.available2019-06-24T13:40:01Z
dc.date.issued2018-12-03
dc.description.abstractMicroRNAs are a class of small non-coding RNA that regulate gene expression at a posttranscriptional level. MicroRNAs have been identified in various body fluids under normal conditions and their stability as well as their dysregulation in disease has led to ongoing interest in their diagnostic and prognostic potential. Circulating microRNAs may be valuable predictors of early-life complications such as birth asphyxia or neonatal seizures but there are relatively few data on microRNA content in plasma from healthy babies. Here we performed small RNA-sequencing analysis of plasma processed from umbilical cord blood in a set of healthy newborns. MicroRNA levels in umbilical cord plasma of four male and four female healthy babies, from two different centres were profiled. A total of 1,004 individual microRNAs were identified, which ranged from 426 to 659 per sample, of which 269 microRNAs were common to all eight samples. Many of these microRNAs are highly expressed and consistent with previous studies using other high throughput platforms. While overall microRNA expression did not differ between male and female cord blood plasma, we did detect differentially edited microRNAs in female plasma compared to male. Of note, and consistent with other studies of this type, adenylation and uridylation were the two most prominent forms of editing. Six microRNAs, miR-128-3p, miR-29a-3p, miR-9-5p, miR-218-5p, 204-5p and miR-132-3p were consistently both uridylated and adenylated in female cord blood plasma. These results provide a benchmark for microRNA profiling and biomarker discovery using umbilical cord plasma and can be used as comparative data for future biomarker profiles from complicated births or those with earlylife developmental disordersen
dc.description.sponsorshipScience Foundation Ireland (SFI/16/RC/3948; SFI/12/COEN/18) ; Health Research Board (HRB Clinician Scientist Award CSA 2012/40)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0207952en
dc.identifier.citationBrennan, G.P., Vitsios, D.M., Casey, S., Looney, A.M., Hallberg, B., Henshall, D.C., Boylan, G.B., Murray, D.M. and Mooney, C., 2018. RNA-sequencing analysis of umbilical cord plasma microRNAs from healthy newborns. PLoS ONE 13(12): e020795, (26pp.) DOI: 10.1371/journal.pone.0207952en
dc.identifier.doi10.1371/journal.pone.0207952en
dc.identifier.eissn1932-6203
dc.identifier.endpage26en
dc.identifier.issued12en
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8100
dc.identifier.volume13en
dc.language.isoenen
dc.publisherPLoS ONEen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2272/IE/Irish Centre for Fetal and Neonatal Translational Research (INFANT)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1891/IE/MicroRNA biofluid profiles as molecular diagnostics for epilepsy/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Advance Award Programme/14/ADV/RC2721/IE/Bioinformatics and predictive analytics for the identification of microRNA biomarkers of neonatal seizures/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-RI/707530/EU/A comprehensive analysis of microRNA-124 regulated gene networks and its role in epilepsy./EpiMIRgenen
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207952
dc.rights© 2018 Brennan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectMicroRNAen
dc.subjectGene expressionen
dc.subjectRNA sequencing analysisen
dc.subjectUmbilical cord plasmaen
dc.titleRNA-sequencing analysis of umbilical cord plasma microRNAs from healthy newbornsen
dc.typeArticle (peer-reviewed)en
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