Phageome analysis of bifidobacteria-rich samples

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Date
03/03/2021
Authors
McDonnell, Brian
Casey, Eoghan
Milani, Christian
Lugli, Gabriele Andrea
Viappiani, Alice
Mahony, Jennifer
Ventura, Marco
van Sinderen, Douwe
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Springer Science+Business Media, LLC
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Abstract
Bifidobacteria are important early colonizers of the human intestinal tract. The relative abundance of bifidobacterial species may be modulated, in part, by bacteriophage activity. Metagenomic studies of these populations is a crucial step in understanding this important interaction. This chapter outlines the technical instructions required to analyze the virome of a bifidobacteria-rich sample, for example, an infant fecal sample.
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Fecal , Phage , Metagenome , DNA , Sequencing
Citation
McDonnell, B., Casey, E., Milani, C., Lugli, G. A., Viappiani, A., Mahony, J., Ventura, M. and van Sinderen, D. (2021) 'Phageome analysis of bifidobacteria-rich samples' in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 71-85. New York, NY: Humana Press. doi: 10.1007/978-1-0716-1274-3_7
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© 2021, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in van Sinderen, D. and Ventura, M. (eds) Bifidobacteria - Methods and Protocols. Methods in Molecular Biology, vol 2278, pp. 71-85. New York, NY: Humana Press. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-1274-3_7