Starch based systems for the colonic delivery of bioactive peptides

dc.check.embargoformatEmbargo not applicable (If you have not submitted an e-thesis or do not want to request an embargo)en
dc.check.infoNot applicableen
dc.check.opt-outNot applicableen
dc.check.reasonNot applicableen
dc.check.typeNo Embargo Required
dc.contributor.advisorBrodkorb, Andréen
dc.contributor.advisorRea, Mary C.en
dc.contributor.advisorMiao, Songen
dc.contributor.advisorHill, Colinen
dc.contributor.authorGough, Ronan
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderTeagascen
dc.date.accessioned2018-08-02T09:33:22Z
dc.date.available2018-08-02T09:33:22Z
dc.date.issued2018
dc.date.submitted2018
dc.description.abstractBioactive peptides have numerous health benefits, although if taken orally they may be digested during gastrointestinal (GI) transit. Encapsulation is an established method for oral delivery of bioactives. However, many current approaches arise from pharmaceutical applications and may be unsuitable for food due to the materials used, cost and scale of production. Therefore, in this project we set out to create a simple and clean-label encapsulation system, suitable for use in the food industry, which could deliver bioactive peptides to the colon. One potential clean-label entrapment material is resistant starch, which is the portion of starch that resists digestion in the upper GI tract but can be digested by bacteria in the colon. As a model bioactive peptide, the well characterised antimicrobial peptide nisin was used; this peptide is normally digested during GI transit. To prepare the nisin a simple purification process was developed, which produced a powder containing ∼33% nisin from a nisin producing culture and also enriched a commercial nisin preparation over 30-fold to a purity of ∼58%. The digestion of nisin was characterised (in vitro) and 6 nisin fragments (4 of which are bioactive) were identified in the digestion products; it was also observed that nisin formed a complex with bile salts that effected its digestion. Nisin was entrapped in starch through multiple approaches based on spray coating, co-spray drying and gel entrapment. A simple approach based on gel entrapment was the most successful and it was shown in vitro to be capable of protecting a portion of the entrapped nisin during transit in the upper GI tract. Using a murine model, it was determined in vivo that a nisin entrapped in starch gel diet significantly (p < 0.001, n = 10) affected the relative abundance of 3 times as many genera from the lower GI tract than a control nisin in starch diet, despite the mice consuming 3-fold less nisin than the control diet.en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGough, R. 2018. Starch based systems for the colonic delivery of bioactive peptides. PhD Thesis, University College Cork.en
dc.identifier.endpage211en
dc.identifier.urihttps://hdl.handle.net/10468/6564
dc.language.isoenen
dc.publisherUniversity College Corken
dc.relation.projectDepartment of Agriculture, Food and the Marine (grant number 10/RD/TMFRC/701); Teagasc (Walsh Fellowship Scheme, grant number 2012221)en
dc.rights© 2018, Ronan Gough.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectColonic deliveryen
dc.subjectBioactive peptidesen
dc.subjectNisinen
dc.subjectStarchen
dc.thesis.opt-outfalse
dc.titleStarch based systems for the colonic delivery of bioactive peptidesen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhDen
ucc.workflow.supervisorc.hill@ucc.ie
Files
Original bundle
Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
GoughR_ThesisAbstract2018.pdf
Size:
110.36 KB
Format:
Adobe Portable Document Format
Description:
Abstract
Loading...
Thumbnail Image
Name:
GoughR_PhD2018.pdf
Size:
4.04 MB
Format:
Adobe Portable Document Format
Description:
Full Text E-thesis
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
5.62 KB
Format:
Item-specific license agreed upon to submission
Description: