Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts

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dc.contributor.authorPayelleville, Amaury
dc.contributor.authorBlackburn, Dana
dc.contributor.authorLanois, Anne
dc.contributor.authorPagès, Sylvie
dc.contributor.authorCambon, Marine C.
dc.contributor.authorGinibre, Nadege
dc.contributor.authorClarke, David J.
dc.contributor.authorGivaudan, Alain
dc.contributor.authorBrillard, Julien
dc.contributor.funderMinistère de l'Enseignement supérieur, de la Recherche et de l'Innovationen
dc.contributor.funderAgence Nationale de la Rechercheen
dc.contributor.funderSociety of Petroleum Engineersen
dc.contributor.funderAcadémie d'agriculture de Francefr
dc.date.accessioned2019-10-26T06:49:47Z
dc.date.available2019-10-26T06:49:47Z
dc.date.issued2019-10-09
dc.description.abstractPhotorhabdus luminescens is an entomopathogenic bacterium found in symbiosis with the nematode Heterorhabditis. Dam DNA methylation is involved in the pathogenicity of many bacteria, including P. luminescens, whereas studies about the role of bacterial DNA methylation during symbiosis are scarce. The aim of this study was to determine the role of Dam DNA methylation in P. luminescens during the whole bacterial life cycle including during symbiosis with H. bacteriophora. We constructed a strain overexpressing dam by inserting an additional copy of the dam gene under the control of a constitutive promoter in the chromosome of P. luminescens and then achieved association between this recombinant strain and nematodes. The dam overexpressing strain was able to feed the nematode in vitro and in vivo similarly as a control strain, and to re-associate with Infective Juvenile (IJ) stages in the insect. No difference in the amount of emerging IJs from the cadaver was observed between the two strains. Compared to the nematode in symbiosis with the control strain, a significant increase in LT50 was observed during insect infestation with the nematode associated with the dam overexpressing strain. These results suggest that during the life cycle of P. luminescens, Dam is not involved the bacterial symbiosis with the nematode H. bacteriophora, but it contributes to the pathogenicity of the nemato-bacterial complex.en
dc.description.sponsorshipGAIA doctoral school (#584);Académie d’Agriculture de France (Dufrenoy Grant); INRA Plant Health and Environment (Society of Petroleum Engineers, SPE-IB17-DiscriMet); French ministries MEAE and MESRI (PHC ULYSSES 2018 grant); French National Research Agency (EPI-PATH project, ANR-17-CE20-0005)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0212655en
dc.identifier.citationPayelleville, A., Blackburn, D., Lanois, A., Pagès, S., Cambon, M. C., Ginibre, N., Clarke, D. J., Givaudan, A. and Brillard, J. (2019) 'Role of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hosts', PLOS ONE, 14(10), e0212655. (14pp.) DOI: 10.1371/journal.pone.0212655en
dc.identifier.doi10.1371/journal.pone.0212655en
dc.identifier.eissn1932-6203
dc.identifier.endpage14en
dc.identifier.issued10en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8872
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212655
dc.rights©2019 Payelleville et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSymbiosisen
dc.subjectDNA methylationen
dc.subjectPathogenesisen
dc.subjectBacterial pathogensen
dc.subjectInsectsen
dc.subjectLarvaeen
dc.subjectNematode infectionsen
dc.subjectPlasmid constructionen
dc.titleRole of the Photorhabdus Dam methyltransferase during interactions with its invertebrate hostsen
dc.typeArticle (peer-reviewed)en
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