Neurotrophic effects of growth/differentiation factor 5 in a neuronal cell line

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dc.contributor.authorToulouse, André
dc.contributor.authorCollins, Grace C.
dc.contributor.authorSullivan, Aideen M.
dc.contributor.funderHigher Education Authorityen
dc.date.accessioned2012-11-08T10:25:17Z
dc.date.available2012-11-08T10:25:17Z
dc.date.copyright2011
dc.date.issued2012
dc.date.updated2012-11-07T09:32:21Z
dc.description.abstractThe neurotrophin growth/differentiation factor 5 (GDF5) is studied as a potential therapeutic agent for Parkinson's disease as it is believed to play a role in the development and maintenance of the nigrostriatal system. Progress in understanding the effects of GDF5 on dopaminergic neurones has been hindered by the use of mixed cell populations derived from primary cultures or in vivo experiments, making it difficult to differentiate between direct and indirect effects of GDF5 treatment on neurones. In an attempt to establish an useful model to study the direct neuronal influence of GDF5, we have characterised the effects of GDF5 on a human neuronal cell line, SH-SY5Y. Our results show that GDF5 has the capability to promote neuronal but not dopaminergic differentiation. We also show that it promotes neuronal survival in vitro following a 6-hydroxydopamine insult. Our results show that application of GDF5 to SH-SY5Y cultures induces the SMAD pathway which could potentially be implicated in the intracellular transmission of GDF5 s neurotrophic effects. Overall, our study shows that the SH-SY5Y neuroblastoma cell line provides an excellent neuronal model to study the neurotrophic effects of GDF5.en
dc.description.sponsorshipPRTLI3 programmeen
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationToulouse, A., Collins, G. & Sullivan, A. (2012). 'Neurotrophic Effects of Growth/Differentiation Factor 5 in a Neuronal Cell Line'. Neurotoxicity Research, 21(3):256-265. http://dx.doi.org/10.1007/s12640-011-9266-7en
dc.identifier.doi10.1007/s12640-011-9266-7
dc.identifier.endpage265en
dc.identifier.issn1029-8428
dc.identifier.issued3en
dc.identifier.journaltitleNeurotoxicity Researchen
dc.identifier.startpage256en
dc.identifier.urihttps://hdl.handle.net/10468/738
dc.identifier.volume21en
dc.language.isoenen
dc.publisherSpringer-Verlagen
dc.rights© 2011, Springer-Verlag. The final publication is available at www.springerlink.comen
dc.subjectGrowth/differentiation factor 5en
dc.subjectNeurotrophinen
dc.subject6-hydroxydopamineen
dc.subjectSH-SY5Y cellsen
dc.subjectParkinson’s diseaseen
dc.titleNeurotrophic effects of growth/differentiation factor 5 in a neuronal cell lineen
dc.typeArticle (peer-reviewed)en
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