Effects of therapeutic hypothermia on the gut microbiota and metabolome of infants suffering hypoxic-ischemic encephalopathy at birth

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dc.check.date2018-10-09
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisheren
dc.contributor.authorWatkins, Claire
dc.contributor.authorMurphy, Kiera
dc.contributor.authorYen, S.
dc.contributor.authorCarafa, Ilaria
dc.contributor.authorDempsey, Eugene M.
dc.contributor.authorO'Shea, Carol Anne
dc.contributor.authorVercoe, E.A.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorStanton, Catherine
dc.contributor.authorRyan, Anthony C.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2018-05-15T12:34:20Z
dc.date.available2018-05-15T12:34:20Z
dc.date.issued2017-10-09
dc.date.updated2018-05-15T11:45:42Z
dc.description.abstractNeonatal hypoxic ischemic encephalopathy (HIE) in the perinatal period can lead to significant neurological deficits in later life. Total body cooling (TBC) is a neuroprotective strategy used in the treatment of HIE and has been shown to reduce seizures and improve neurodevelopmental outcomes in treated infants. Little is known, however, about the effects of HIE/TBC on the developing gut microbiota composition and subsequent metabolic profile. Ten term infants with HIE who received TBC at 33.5 degrees C for 72 h were recruited. A control group consisted of nine healthy full term infants. Faecal samples were collected from both groups at 2 years of age and stored at -20 degrees C. 16S rRNA amplicon Illumina sequencing was carried out to determine gut microbiota composition and 1H NMR analysis was performed to determine the metabolic profile of faecal water. The gut microbiota composition of the HIE/TBC infants were found to have significantly lower proportions of Bacteroides compared to the non-cooled healthy control group. Alpha diversity measures detected significantly lower diversity in microbial richness in the HIE/TBC infant group compared to the control infants (Shannon index,en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (Food Institutional Research Measure (FIRM); (INFANTMET)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationWatkins, C., Murphy, K., Yen, S., Carafa, I., Dempsey, E. M., O’Shea, C. A., Vercoe, E. A., Ross, R. P., Stanton, C. and Ryan, C. A. (2017) 'Effects of therapeutic hypothermia on the gut microbiota and metabolome of infants suffering hypoxic-ischemic encephalopathy at birth', The International Journal of Biochemistry & Cell Biology, 93, pp. 110-118. doi:10.1016/j.biocel.2017.08.017en
dc.identifier.doi10.1016/j.biocel.2017.08.017
dc.identifier.endpage118en
dc.identifier.issn1357-2725
dc.identifier.journaltitleThe International Journal of Biochemistry & Cell Biologyen
dc.identifier.startpage110en
dc.identifier.urihttps://hdl.handle.net/10468/6108
dc.identifier.volume93en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://doi.org/10.1016/j.biocel.2019.05.019
dc.rights© 2017, Elsevier Ltd. All rights reserved.This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectHuman breast-milken
dc.subjectNeonatal encephalopathyen
dc.subjectNecrotizing enterocolitisen
dc.subjectBrainen
dc.subjectMechanismsen
dc.subjectFormulasen
dc.subjectHealthen
dc.subjectRatsen
dc.titleEffects of therapeutic hypothermia on the gut microbiota and metabolome of infants suffering hypoxic-ischemic encephalopathy at birthen
dc.typeArticle (peer-reviewed)en
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