In-vitro study of the effect of anti-hypertensive drugs on placental hormones and angiogenic proteins synthesis in pre-eclampsia

Simple item page
dc.contributor.authorGangooly, Subrata
dc.contributor.authorMuttukrishna, Shanthi
dc.contributor.authorJauniaux, Eric
dc.date.accessioned2016-02-17T11:43:38Z
dc.date.available2016-02-17T11:43:38Z
dc.date.issued2014
dc.description.abstractIntroduction: Antihypertensive drugs lower the maternal blood pressure in pre-eclampsia (PE) by direct or central vasodilatory mechanisms but little is known about the direct effects of these drugs on placental functions. Objective: The aim of our study is to evaluate the effect of labetolol, hydralazine, a-methyldopa and pravastatin on the synthesis of placental hormonal and angiogenic proteins know to be altered in PE. Design: Placental villous explants from late onset PE (n = 3) and normotensive controls (n = 6) were cultured for 3 days at 10 and 20% oxygen (O-2) with variable doses anti-hypertensive drugs. The levels of activin A, inhibin A, human Chorionic Gonadotrophin (hCG), soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) were measured in explant culture media on day 1, 2 and 3 using standard immunoassays. Data at day 1 and day 3 were compared. Results: Spontaneous secretion of sEndoglin and sFlt-1 were higher (p<0.05) in villous explants from PE pregnancies compared to controls. There was a significant time dependant decrease in the secretion of sFlt-1 and sEndoglin in PE cases, which was seen only for sFlt-1 in controls. In both PE cases and controls the placental protein secretions were not affected by varying doses of anti-hypertensive drugs or the different O-2 concentration cultures, except for Activin, A which was significantly (p<0.05) higher in controls at 10% O-2. Interpretation: Our findings suggest that the changes previously observed in maternal serum hormones and angiogenic proteins level after anti-hypertensive treatment in PE could be due to a systemic effect of the drugs on maternal blood pressure and circulation rather than a direct effect of these drugs on placental biosynthesis and/or secretion.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide107644
dc.identifier.citationGangooly S, Muttukrishna S, Jauniaux E (2014) In-Vitro Study of the Effect of Anti-Hypertensive Drugs on Placental Hormones and Angiogenic Proteins Synthesis in Pre-Eclampsia. PLoS ONE 9(9): e107644. doi:10.1371/journal.pone.0107644
dc.identifier.doi10.1371/journal.pone.0107644
dc.identifier.issn1932-6203
dc.identifier.issued9en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2324
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Gangooly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectHuman early pregnancyen
dc.subjectTyrosine kinase 1en
dc.subjectOxidative stressen
dc.subjectActivin Aen
dc.subjectHypertensive disordersen
dc.subjectSoluble endoglinen
dc.subjectBlood flowen
dc.subjectInhibinen
dc.subjectAgentsen
dc.subjectOxygenen
dc.titleIn-vitro study of the effect of anti-hypertensive drugs on placental hormones and angiogenic proteins synthesis in pre-eclampsiaen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
SG_In-VitroPV2014.pdf
Size:
288.03 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Obstetrics & Gynaecology - Journal articles