Resazurin-based assay for quantifying living cells during alkaline phosphatase (ALP) release

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dc.contributor.authorBalbaied, Thanih
dc.contributor.authorMoore, Eric
dc.contributor.funderMinistry of Education – Kingdom of Saudi Arabien
dc.date.accessioned2020-11-13T16:08:56Z
dc.date.available2020-11-13T16:08:56Z
dc.date.issued2020-05-31
dc.date.updated2020-11-13T15:51:12Z
dc.description.abstractAlkaline phosphatase (ALP) is an important reporter gene in the gene expression system, therefore monitoring cellular behavior including cell viability during ALP release is of significance. This assay produced a quantitative resazurin-based assay for cell viability in embryonic and cancer cells during alkaline phosphatase (ALP) release. A post-confluence culture method was applied to induce ALP in the cells of Balb/c 3T3, A549, MCF-7, and Ht-29. The density of each cell type was optimized using the standard cell culture assay. The main parameters affecting the results of resazurin involve the concentration of resazurin, incubation time, and cell number. The redox reaction, in which resazurin is reduced by the cells, was measured by fluorescence at 544 nm and 590 nm. The obtained data were compared with the hemocytometer assay. ALP release was determined using the optical active substrate p-nitrophenyl phosphate and colorimetric assay.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid3840en
dc.identifier.citationBalbaied, T. and Moore, E. (2020) 'Resazurin-Based Assay for Quantifying Living Cells during Alkaline Phosphatase (ALP) Release', Applied Sciences, 10(11), 3840 (16 pp). doi: 10.3390/app10113840en
dc.identifier.doi10.3390/app10113840en
dc.identifier.endpage16en
dc.identifier.issn2076-3417
dc.identifier.issued11en
dc.identifier.journaltitleApplied Sciences-Baselen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/10755
dc.identifier.volume10en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.mdpi.com/2076-3417/10/11/3840
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectResazurin-based assaysen
dc.subjectCell viabilityen
dc.subjectAlkaline phosphataseen
dc.subjectBalb/c 3T3 cellsen
dc.subjectA549 cellsen
dc.subjectMCF-7 cellsen
dc.titleResazurin-based assay for quantifying living cells during alkaline phosphatase (ALP) releaseen
dc.typeArticle (peer-reviewed)en
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