Biotechnological potential of cold adapted Pseudoalteromonas spp. isolated from "deep sea' sponges

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dc.contributor.authorBorchert, Erik
dc.contributor.authorKnobloch, Stephen
dc.contributor.authorDwyer, Emilie
dc.contributor.authorFlynn, Sinead
dc.contributor.authorJackson, Stephen A.
dc.contributor.authorJohannsson, Ragnar
dc.contributor.authorMarteinsson, Viggo T.
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorDobson, Alan D. W.
dc.contributor.funderMarine Institute
dc.contributor.funderEuropean Social Fund
dc.contributor.funderSeventh Framework Programme
dc.date.accessioned2017-09-26T11:39:19Z
dc.date.available2017-09-26T11:39:19Z
dc.date.issued2017
dc.description.abstractThe marine genus Pseudoalteromonas is known for its versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest. We have sequenced the genomes of three Pseudoalteromonas sp. strains isolated from different deep sea sponges on the Illumina MiSeq platform. The isolates have been screened for various industrially important enzymes and comparative genomics has been applied to investigate potential relationships between the isolates and their host organisms, while comparing them to free-living Pseudoalteromonas spp. from shallow and deep sea environments. The genomes of the sponge associated Pseudoalteromonas strains contained much lower levels of potential eukaryotic-like proteins which are known to be enriched in symbiotic sponge associated microorganisms, than might be expected for true sponge symbionts. While all the Pseudoalteromonas shared a large distinct subset of genes, nonetheless the number of unique and accessory genes is quite large and defines the pan-genome as open. Enzymatic screens indicate that a vast array of enzyme activities is expressed by the isolates, including -galactosidase, -glucosidase, and protease activities. A -glucosidase gene from one of the Pseudoalteromonas isolates, strain EB27 was heterologously expressed in Escherichia coli and, following biochemical characterization, the recombinant enzyme was found to be cold-adapted, thermolabile, halotolerant, and alkaline active.en
dc.description.sponsorshipMarine Institute (Beaufort Marine Research Award)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid184
dc.identifier.citationBorchert, E., Knobloch, S., Dwyer, E., Flynn, S., Jackson, S. A., Jóhannsson, R., Marteinsson, V. T., O’Gara, F. and Dobson, A. D. W. (2017) 'Biotechnological potential of cold adapted Pseudoalteromonas spp. isolated from "deep sea' sponges', Marine Drugs, 15(6), (17pp). doi: 10.3390/md15060184en
dc.identifier.doi10.3390/md15060184
dc.identifier.issn1660-3397
dc.identifier.issued6
dc.identifier.journaltitleMarine Drugsen
dc.identifier.urihttps://hdl.handle.net/10468/4785
dc.identifier.volume15
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA
dc.relation.urihttp://www.mdpi.com/1660-3397/15/6/184
dc.rights© 2017, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPseudoalteromonasen
dc.subjectEnzymesen
dc.subjectGenomicsen
dc.subjectPan genomejoint genome instituteen
dc.subjectPilot scale productionen
dc.subjectSingle cell genomicsen
dc.subjectMarine bacteriumen
dc.subjectSp.noven
dc.subjectBiochemical characterizationen
dc.subjectSuperoxide dismutaseen
dc.subjectRapid annotationen
dc.subjectInteractive treeen
dc.subjectGene clustersen
dc.titleBiotechnological potential of cold adapted Pseudoalteromonas spp. isolated from "deep sea' spongesen
dc.typeArticle (peer-reviewed)en
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