Biotechnological potential of cold adapted Pseudoalteromonas spp. isolated from "deep sea' sponges
dc.contributor.author | Borchert, Erik | |
dc.contributor.author | Knobloch, Stephen | |
dc.contributor.author | Dwyer, Emilie | |
dc.contributor.author | Flynn, Sinead | |
dc.contributor.author | Jackson, Stephen A. | |
dc.contributor.author | Johannsson, Ragnar | |
dc.contributor.author | Marteinsson, Viggo T. | |
dc.contributor.author | O'Gara, Fergal | |
dc.contributor.author | Dobson, Alan D. W. | |
dc.contributor.funder | Marine Institute | |
dc.contributor.funder | European Social Fund | |
dc.contributor.funder | Seventh Framework Programme | |
dc.date.accessioned | 2017-09-26T11:39:19Z | |
dc.date.available | 2017-09-26T11:39:19Z | |
dc.date.issued | 2017 | |
dc.description.abstract | The marine genus Pseudoalteromonas is known for its versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest. We have sequenced the genomes of three Pseudoalteromonas sp. strains isolated from different deep sea sponges on the Illumina MiSeq platform. The isolates have been screened for various industrially important enzymes and comparative genomics has been applied to investigate potential relationships between the isolates and their host organisms, while comparing them to free-living Pseudoalteromonas spp. from shallow and deep sea environments. The genomes of the sponge associated Pseudoalteromonas strains contained much lower levels of potential eukaryotic-like proteins which are known to be enriched in symbiotic sponge associated microorganisms, than might be expected for true sponge symbionts. While all the Pseudoalteromonas shared a large distinct subset of genes, nonetheless the number of unique and accessory genes is quite large and defines the pan-genome as open. Enzymatic screens indicate that a vast array of enzyme activities is expressed by the isolates, including -galactosidase, -glucosidase, and protease activities. A -glucosidase gene from one of the Pseudoalteromonas isolates, strain EB27 was heterologously expressed in Escherichia coli and, following biochemical characterization, the recombinant enzyme was found to be cold-adapted, thermolabile, halotolerant, and alkaline active. | en |
dc.description.sponsorship | Marine Institute (Beaufort Marine Research Award) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.articleid | 184 | |
dc.identifier.citation | Borchert, E., Knobloch, S., Dwyer, E., Flynn, S., Jackson, S. A., Jóhannsson, R., Marteinsson, V. T., O’Gara, F. and Dobson, A. D. W. (2017) 'Biotechnological potential of cold adapted Pseudoalteromonas spp. isolated from "deep sea' sponges', Marine Drugs, 15(6), (17pp). doi: 10.3390/md15060184 | en |
dc.identifier.doi | 10.3390/md15060184 | |
dc.identifier.issn | 1660-3397 | |
dc.identifier.issued | 6 | |
dc.identifier.journaltitle | Marine Drugs | en |
dc.identifier.uri | https://hdl.handle.net/10468/4785 | |
dc.identifier.volume | 15 | |
dc.language.iso | en | en |
dc.publisher | MDPI AG | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN | |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA | |
dc.relation.uri | http://www.mdpi.com/1660-3397/15/6/184 | |
dc.rights | © 2017, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Pseudoalteromonas | en |
dc.subject | Enzymes | en |
dc.subject | Genomics | en |
dc.subject | Pan genomejoint genome institute | en |
dc.subject | Pilot scale production | en |
dc.subject | Single cell genomics | en |
dc.subject | Marine bacterium | en |
dc.subject | Sp.nov | en |
dc.subject | Biochemical characterization | en |
dc.subject | Superoxide dismutase | en |
dc.subject | Rapid annotation | en |
dc.subject | Interactive tree | en |
dc.subject | Gene clusters | en |
dc.title | Biotechnological potential of cold adapted Pseudoalteromonas spp. isolated from "deep sea' sponges | en |
dc.type | Article (peer-reviewed) | en |