PBP2b mutations improve the growth of phage-resistant Lactococcus cremoris lacking polysaccharide pellicle

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dc.contributor.authorGuérin, Hugoen
dc.contributor.authorQuénée, Pascalen
dc.contributor.authorPalussière, Simonen
dc.contributor.authorCourtin, Pascalen
dc.contributor.authorAndré, Gwenaëlleen
dc.contributor.authorPéchoux, Christineen
dc.contributor.authorCostache, Vladen
dc.contributor.authorMahony, Jenniferen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorKulakauskas, Sauliusen
dc.contributor.authorChapot-Chartier, Marie-Pierreen
dc.contributor.editorCharles M. Dozoisen
dc.date.accessioned2023-08-10T09:01:18Z
dc.date.available2023-08-10T09:01:18Z
dc.date.issued2023-05-24en
dc.description.abstractLactococcus lactis and Lactococcus cremoris are Gram-positive lactic acid bacteria widely used as starter in milk fermentations. Lactococcal cells are covered with a polysaccharide pellicle (PSP) that was previously shown to act as the receptor for numerous bacteriophages of the Caudoviricetes class. Thus, mutant strains lacking PSP are phage resistant. However, because PSP is a key cell wall component, PSP-negative mutants exhibit dramatic alterations of cell shape and severe growth defects, which limit their technological value. In the present study, we isolated spontaneous mutants with improved growth, from L. cremoris PSP-negative mutants. These mutants grow at rates similar to the wild-type strain, and based on transmission electron microscopy analysis, they exhibit improved cell morphology compared to their parental PSP-negative mutants. In addition, the selected mutants maintain their phage resistance. Whole-genome sequencing of several such mutants showed that they carried a mutation in pbp2b, a gene encoding a penicillin-binding protein involved in peptidoglycan biosynthesis. Our results indicate that lowering or turning off PBP2b activity suppresses the requirement for PSP and ameliorates substantially bacterial fitness and morphology.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGuérin, H., Quénée, P., Palussière, S., Courtin, P., André, G., Péchoux, C., Costache, V., Mahony, J., van Sinderen, D., Kulakauskas, S. and Chapot-Chartier, M. P. (2023) 'PBP2b mutations improve the growth of phage-resistant Lactococcus cremoris lacking polysaccharide pellicle', Applied and Environmental Microbiology, 89(6), e02103-22 (14pp). doi: 10.1128/aem.02103-22en
dc.identifier.doi10.1128/aem.02103-22en
dc.identifier.eissn1098-5336en
dc.identifier.endpage14en
dc.identifier.issn0099-2240en
dc.identifier.issued6en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14808
dc.identifier.volume89en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.rights© 2023, American Society for Microbiology. All Rights Reserved.en
dc.subjectLactococcusen
dc.subjectBacteriophage resistanceen
dc.subjectCell wallen
dc.subjectLactic acid bacteriaen
dc.subjectPenicillin-binding proteinsen
dc.subjectPolysaccharide pellicleen
dc.titlePBP2b mutations improve the growth of phage-resistant Lactococcus cremoris lacking polysaccharide pellicleen
dc.typeArticle (peer-reviewed)en
oaire.citation.issue6en
oaire.citation.volume89en
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