Transcriptional slippage in the positive-sense RNA virus family Potyviridae

Simple item page
dc.contributor.authorOlspert, Allan
dc.contributor.authorChung, Betty Y-W
dc.contributor.authorAtkins, John F.
dc.contributor.authorCarr, John P.
dc.contributor.authorFirth, Andrew E.
dc.contributor.funderWellcome Trusten
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.date.accessioned2018-03-29T11:03:45Z
dc.date.available2018-03-29T11:03:45Z
dc.date.issued2015-06-25
dc.date.updated2018-03-29T10:49:27Z
dc.description.abstractThe family Potyviridae encompasses ~30% of plant viruses and is responsible for significant economic losses worldwide. Recently, a small overlapping coding sequence, termed pipo, was found to be conserved in the genomes of all potyvirids. PIPO is expressed as part of a frameshift protein, P3N‐PIPO, which is essential for virus cell‐to‐cell movement. However, the frameshift expression mechanism has hitherto remained unknown. Here, we demonstrate that transcriptional slippage, specific to the viral RNA polymerase, results in a population of transcripts with an additional “A” inserted within a highly conserved GAAAAAA sequence, thus enabling expression of P3N‐PIPO. The slippage efficiency is ~2% in Turnip mosaic virus and slippage is inhibited by mutations in the GAAAAAA sequence. While utilization of transcriptional slippage is well known in negative‐sense RNA viruses such as Ebola, mumps and measles, to our knowledge this is the first report of its widespread utilization for gene expression in positive‐sense RNA viruses.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationOlspert, A., Chung, B. Y. W., Atkins, J. F., Carr, J. P. and Firth, A. E. (2015) 'Transcriptional slippage in the positive‐sense RNA virus family Potyviridae', EMBO reports, 16(8), pp. 995-1004. doi: 10.15252/embr.201540509en
dc.identifier.doi10.15252/embr.201540509
dc.identifier.endpage1004en
dc.identifier.issn1469-3178
dc.identifier.issued8en
dc.identifier.journaltitleEmbo Reportsen
dc.identifier.startpage995en
dc.identifier.urihttps://hdl.handle.net/10468/5718
dc.identifier.volume16en
dc.language.isoenen
dc.publisherEMBO Pressen
dc.relation.projectinfo:eu-repo/grantAgreement/WT/Immunology and Infectious Disease/088789//Systematic Bioinformatic Discovery of 'Hidden' Virus Genes./en
dc.relation.projectinfo:eu-repo/grantAgreement/WT/Immunology and Infectious Disease/106207//Encyclopedia of RNA Virus Elements: systematic bioinformatic discovery and experimental analysis of hidden genes, non-canonical translation mechanisms, and other functional elements in RNA virus genomes./en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/J007072/1/GB/Non-canonical gene expression: Investigating a novel stimulator and a novel function for ribosomal frameshifting/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/J015652/1/GB/Deciphering the enigmatic expression mechanism of the newly discovered PIPO gene in the Potyviridae family of plant viruses/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/J015652/1/GB/Deciphering the enigmatic expression mechanism of the newly discovered PIPO gene in the Potyviridae family of plant viruses/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/J011762/1/GB/Modelling and manipulation of plant-aphid interactions: A new avenue for sustainable disease management of an important crop in Africa/en
dc.relation.urihttp://embor.embopress.org/content/embor/16/8/995.full.pdf
dc.rights© 2015 The Authors. Published by EMBO Press under the terms of the CC BY 4.0 licenseen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectGene expressionen
dc.subjectP3N-PIPOen
dc.subjectPotyvirusen
dc.subjectRNA virusen
dc.subjectTranscriptional slippageen
dc.titleTranscriptional slippage in the positive-sense RNA virus family Potyviridaeen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
5102.pdf
Size:
979.72 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Biochemistry and Cell Biology - Journal Articles