Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet

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dc.contributor.authorTan, Si
dc.contributor.authorCaparros-Martin, Jose A.
dc.contributor.authorMatthews, Vance B.
dc.contributor.authorKoch, Henrietta
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorCroft, Kevin D.
dc.contributor.authorWard, Natalie C.
dc.contributor.funderCurtin University of Technology
dc.date.accessioned2018-08-29T15:47:26Z
dc.date.available2018-08-29T15:47:26Z
dc.date.issued2018
dc.description.abstractDietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites. These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters. The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome. Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10). Body weight and food intake were measured weekly. At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected. At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice. Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet. Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids. In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet. This protective effect appears to be mediated, in part, through beneficial changes to the microbiome.en
dc.description.sponsorshipCurtin University of Technology (Faculty of Health Science)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid10100
dc.identifier.citationTan, S., Caparros-Martin, J. A., Matthews, V. B., Koch, H., O’Gara, F., Croft, K. D. and Ward, N. C. (2018) 'Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet', Scientific Reports, 8(1), 10100 (13pp). doi: 10.1038/s41598-018-28521-8en
dc.identifier.doi10.1038/s41598-018-28521-8
dc.identifier.endpage13
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6673
dc.identifier.volume8
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urihttps://www.nature.com/articles/s41598-018-28521-8
dc.rights© 2018, the Authors. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMarker gene-sequencesen
dc.subjectGrowth-factor 21en
dc.subjectE-knockout miceen
dc.subjectEndothelial dysfunctionen
dc.subjectInsulin-resistanceen
dc.subjectHeme oxygenase-1en
dc.subjectFood-intakeen
dc.subjectObesityen
dc.subjectHealthen
dc.subjectFiberen
dc.titleIsoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat dieten
dc.typeArticle (peer-reviewed)en
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