Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna

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dc.contributor.authorRainville, Louis-Charles
dc.contributor.authorCarolan, Darragh
dc.contributor.authorVarela, Ana Coelho
dc.contributor.authorDoyle, Hugh
dc.contributor.authorSheehan, David
dc.contributor.funderHigher Education Authorityen
dc.contributor.funderIrish Research Council for Science Engineering and Technologyen
dc.contributor.funderFonds Québécois de la Recherche sur la Nature et les Technologies, Canadafr
dc.date.accessioned2014-08-27T09:01:21Z
dc.date.available2015-01-17T05:00:05Z
dc.date.issued2014-01
dc.date.updated2014-08-01T15:57:16Z
dc.description.abstractRecent decades have seen a strong increase in the promise and uses of nanotechnology. This is correlated with their growing release in the environment and there is concern that nanomaterials may endanger ecosystems. Silver nanoparticles (AgNPs) have some of the most varied applications, making their release into the environment unavoidable. In order to assess their potential toxicity in aquatic environments, the acute toxicity of citrate-coated AgNPs to Daphnia magna was measured and compared to that of AgNO3. AgNPs were found to be ten times less toxic by mass than silver ions, and most of this toxicity was removed by ultracentrifuging. At the protein level, the two forms of silver had different impacts. Both increased protein thiol content, while only AgNP increased carbonyl levels. In 2DE of samples labelled for carbonyls, no feature was significantly affected by both compounds, indicating different modes of toxicity. Identified proteins showed functional overlap between the two compounds: vitellogenins (vtg) were present in most features identified, indicating their role as a general stress sensor. In addition to vtg, hemoglobin levels were increased by the AgNP exposure while 14-3-3 protein (a regulatory protein) carbonylation levels were reduced by AgNO3. Overall, this study confirms the previously observed lower acute toxicity of AgNPs, while demonstrating that the toxicity of both forms of silver follow somewhat different biologic pathways, potentially leading to different interactions with natural compounds or pollutants in the aquatic environment.en
dc.description.sponsorshipHigher Education Authority (PRTLI program (Cycle 3 Nanoscience and Cycle 4 INSPIRE))en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRAINVILLE, L.-C., CAROLAN, D., VARELA, A. C., DOYLE, H. & SHEEHAN, D. 2014. Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna. Analyst, 139, 1678-1686. doi: 10.1039/C3AN02160Ben
dc.identifier.doi10.1039/c3an02160b
dc.identifier.endpage1686en
dc.identifier.issn0003-2654
dc.identifier.issued7en
dc.identifier.journaltitleAnalysten
dc.identifier.startpage1678en
dc.identifier.urihttps://hdl.handle.net/10468/1631
dc.identifier.volume139en
dc.language.isoenen
dc.publisherThe Royal Society of Chemistryen
dc.relation.urihttp://pubs.rsc.org/en/content/articlepdf/2014/an/c3an02160b
dc.rights© The Royal Society of Chemistry 2014. Reproduced by permission of The Royal Society of Chemistry.en
dc.subjectAnalytical toxicology of nanoparticlesen
dc.subjectNanoparticle toxicityen
dc.subjectSilver nitrate toxicityen
dc.subjectCitrate-coated AgNPen
dc.titleProteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magnaen
dc.typeArticle (peer-reviewed)en
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