The role of biofluorescence in the health and welfare of integrated multi-trophic aquaculture species

Juhasz-Dora, Thomas

The role of biofluorescence in the health and welfare of integrated multi-trophic aquaculture species

dc.check.chapterOfThesis	Chapter 9 as it is not yet published.	en
dc.check.info	Partial Restriction
dc.contributor.advisor	Doyle, Thomas
dc.contributor.advisorexternal	Maguire, Julie
dc.contributor.author	Juhasz-Dora, Thomas	en
dc.contributor.funder	Horizon 2020
dc.date.accessioned	2025-02-10T14:25:43Z
dc.date.available	2025-02-10T14:25:43Z
dc.date.issued	2024
dc.date.submitted	2024
dc.description	Partial Restriction
dc.description.abstract	Biofluorescence in marine organisms is emitted as a lower wavelength light by the fluorophore compounds present within their bodies after excitation by the blue-shifted light of the water column. Biofluorescence produced in healthy scleractinian coral tissue has been applied as a non-invasive monitoring tool for gauging the physiological response of corals to environmental stress and disease outbreaks. By measuring shifting levels of fluorescent emissions in tissue, changes in the physiological status can be identified. However, the presence of and responsiveness of biofluorescence to stress in higher order marine organisms used in aquaculture operations is unknown. The first phase of this thesis assessed whether biofluorescence was produced in seven economically valued species present within the northeast Atlantic Ocean: the commercially valued macroalgal species Laminaria digitata, Palmaria palmata, and Saccharina latissima, the green sea urchin Strongylocentrotus droebachiensis, the European black sea cucumber Holothuria forskali, the red king crab Paralithodes camtschaticus, and the lumpfish Cyclopterus lumpus. Biofluorescence was documented in all species, with the complex emissions produced from physical damage or released exudates in sea urchins, while the lumpfish biofluorescence differed according to life stage and sex. The second phase of this study assessed whether biofluorescence responded to stressors typically experienced in aquaculture. Green sea urchins exposed to three environmental variables (out of water transport, in water transport at elevated temperatures, and in water transport at ambient temperature) produced fluorescent emissions in response to all treatments in their external anatomy but varied in intensity according to treatment type, time interval sampled, and in their coelomic fluid fluorescence. Furthermore, the fluorescence emissions in the red king crab were responsive when subjected to the out of water shipping methodology used for market transport, increasing in both the external anatomy and the haemolymph after exposure. Likewise, lumpfish subjected to a freshwater therapeutic bath used to control parasites increased fluorescent emissions in the green spectra, while the control group placed back into saltwater did not increase in intensity. The identification of reproductive development stages in macroalgae was tested using fluorescence. The exploration of fluorescent emission within the blades found differences in intensity according to whether reproductive sori were present in the blade. Biofluorescence has been found through these studies to be an effective alternative method(s) to non-invasively assess subclinical stress, wounds, and even reproductive status in marine organisms on a real-time basis. The third phase of this thesis was a pharmacokinetic study of European black sea cucumber Holothuria forskali tissue after the animals ingested salmon pellets simultaneously treated with the recommended dosages for the Atlantic salmon therapeutants emamectin benzoate and oxytetracycline. Liquid chromatography with tandem mass spectrometry detected greater bioavailability of oxytetracycline in sea cucumber tissue, requiring 18-34 days for elimination in comparison to the 9-14 days for emamectin benzoate. These results reflect a more realistic scenario of how sea cucumbers react to feeding on organic matter containing veterinary therapeutants, helping to define a baseline for the withdrawal period for the two compounds in sea cucumber tissue as well as for further studies on the dynamics within actual field conditions.	en
dc.description.status	Not peer reviewed	en
dc.description.version	Accepted Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Juhasz-Dora, T. 2024. The role of biofluorescence in the health and welfare of integrated multi-trophic aquaculture species. PhD Thesis, University College Cork.
dc.identifier.endpage	185
dc.identifier.uri	https://hdl.handle.net/10468/17013
dc.language.iso	en	en
dc.publisher	University College Cork	en
dc.relation.project	info:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/956697/EU/European Aquaculture Training for improving Seafood Husbandry/EATFISH
dc.rights	© 2024, Thomas Juhasz-Dora.
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Biofluorescence	en
dc.subject	Lumpfish	en
dc.subject	Red king crab	en
dc.subject	Macroalgae	en
dc.subject	Sea urchins	en
dc.subject	Echinoderms	en
dc.subject	Stress	en
dc.subject	Hyperspectral imaging	en
dc.title	The role of biofluorescence in the health and welfare of integrated multi-trophic aquaculture species
dc.type	Doctoral thesis	en
dc.type.qualificationlevel	Doctoral	en
dc.type.qualificationname	PhD - Doctor of Philosophy	en