Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo

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dc.contributor.authorO'Mahony, Conor
dc.contributor.authorHoulihan, Ruth
dc.contributor.authorGrygoryev, Konstantin
dc.contributor.authorNing, Zhenfei
dc.contributor.authorWilliams, John
dc.contributor.authorMoore, Thomas F.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHigher Education Authorityen
dc.date.accessioned2016-11-28T15:21:37Z
dc.date.available2016-11-28T15:21:37Z
dc.date.issued2016-11
dc.date.updated2016-11-28T15:09:40Z
dc.description.abstractWe analysed the use of microneedle-based electrodes to enhance electroporation of mouse testis with DNA vectors for production of transgenic mice. Different microneedle formats were developed and tested, and we ultimately used electrodes based on arrays of 500 μm tall microneedles. In a series of experiments involving injection of a DNA vector expressing Green Fluorescent Protein (GFP) and electroporation using microneedle electrodes and a commercially available voltage supply, we compared the performance of flat and microneedle electrodes by measuring GFP expression at various timepoints after electroporation. Our main finding, supported by both experimental and simulated data, is that needles significantly enhanced electroporation of testis.en
dc.description.sponsorshipHigher Education Authority (PRTLI5 Irish Transgenic Network infrastructure grant); Science Foundation Ireland (SFI National Access Programme (NAP 374))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO'Mahony, C., Houlihan, R., Grygoryev, K., Ning, Z., Williams, J. and Moore, T. (2016) 'Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo'. Journal of Physics: Conference Series, 757 :012040-1-012040-6. doi: 10.1088/1742-6596/757/1/012040en
dc.identifier.doi10.1088/1742-6596/757/1/012040
dc.identifier.endpage012040-6en
dc.identifier.issn1742-6588,
dc.identifier.journaltitleJournal of Physics: Conference Seriesen
dc.identifier.startpage012040-1en
dc.identifier.urihttps://hdl.handle.net/10468/3315
dc.identifier.volume757en
dc.language.isoenen
dc.publisherIOP Publishingen
dc.rights© 2016, The Authors. Published under licence by IOP Publishing Ltd. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.en
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/en
dc.subjectMicroneedle formatsen
dc.subjectMicroneedle electrodesen
dc.subjectGFP expressionen
dc.subjectElectroporationen
dc.titleDesign, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivoen
dc.typeArticle (peer-reviewed)en
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