Characterization of Heterorhabditis isolates by PCR amplification of segments of mtDNA and rDNA genes

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dc.contributor.authorJoyce, Susan A.
dc.contributor.authorBurnell, Ann M.
dc.contributor.authorPowers, Thomas O.
dc.date.accessioned2018-02-08T15:23:44Z
dc.date.available2018-02-08T15:23:44Z
dc.date.issued1994-09
dc.date.updated2018-02-08T15:20:27Z
dc.description.abstractRestriction digests of amplified DNA from the mitochondrial genome and the nuclear ribosomal internally transcribed spacer region have been evaluated as genetic markers for species groups in Heterorhabditis. Six RFLP profiles have been identified. These profiles supported groupings determined by cross-breeding studies and were in agreement with less definitive groupings based on other biochemical and molecular methods. Digestion patterns of both amplification products provided strong evidence for the recognition of species groups, which include Irish, NW European, tropical, and a H. bacteriophora complex. The H. bacteriophora complex could be further resolved into three genotypes represented by H. zealandica, the H. bacteriophora, Brecon (Australian) type isolate for H. bacteriophora, and a grouping composed of isolates NC1, V16, HI82, and HP88. All cultures obtained of the H. megidis isolate were identical to the NW European group. These results could be used to aid monitoring of field release of Heterorhabditis as well as allowing a rapid initial assessment of taxonomic grouping.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJoyce, S. A., Burnell, A. M. and Powers, T. O. (1994) 'Characterization of Heterorhabditis Isolates by PCR Amplification of Segments of mtDNA and rDNA Genes', Journal of Nematology, 26 (3):260-270. Available at: http://journals.fcla.edu/jon/article/view/66625en
dc.identifier.endpage270en
dc.identifier.issn0022-300X
dc.identifier.issued3en
dc.identifier.journaltitleJournal of Nematologyen
dc.identifier.startpage260en
dc.identifier.urihttps://hdl.handle.net/10468/5428
dc.identifier.volume26en
dc.language.isoenen
dc.publisherSociety of Nematologistsen
dc.relation.urihttp://journals.fcla.edu/jon/article/view/66625
dc.rights© The Society of Nematologists 1994.en
dc.subjectEntomopathogenic nematodeen
dc.subjectHeterorhabditisen
dc.subjectMolecular diagnosticsen
dc.subjectmtDNAen
dc.subjectNematode,en
dc.subjectRibosomal DNAen
dc.titleCharacterization of Heterorhabditis isolates by PCR amplification of segments of mtDNA and rDNA genesen
dc.typeArticle (peer-reviewed)en
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