Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds

Naughton, Lynn M.; Romano, Stefano; O'Gara, Fergal; Dobson, Alan D. W.

Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds

dc.contributor.author	Naughton, Lynn M.
dc.contributor.author	Romano, Stefano
dc.contributor.author	O'Gara, Fergal
dc.contributor.author	Dobson, Alan D. W.
dc.contributor.funder	European Social Fund
dc.contributor.funder	Marine Institute
dc.contributor.funder	Department of Agriculture, Food and the Marine
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	Seventh Framework Programme
dc.contributor.funder	European Commission
dc.date.accessioned	2017-09-26T11:39:22Z
dc.date.available	2017-09-26T11:39:22Z
dc.date.issued	2017
dc.description.abstract	Increased incidences of antimicrobial resistance and the emergence of pan-resistant 'superbugs' have provoked an extreme sense of urgency amongst researchers focusing on the discovery of potentially novel antimicrobial compounds. A strategic shift in focus from the terrestrial to the marine environment has resulted in the discovery of a wide variety of structurally and functionally diverse bioactive compounds from numerous marine sources, including sponges. Bacteria found in close association with sponges and other marine invertebrates have recently gained much attention as potential sources of many of these novel bioactive compounds. Members of the genus Pseudovibrio are one such group of organisms. In this study, we interrogate the genomes of 21 Pseudovibrio strains isolated from a variety of marine sources, for the presence, diversity and distribution of biosynthetic gene clusters (BGCs). We expand on results obtained from antiSMASH analysis to demonstrate the similarity between the Pseudovibriorelated BGCs and those characterized in other bacteria and corroborate our findings with phylogenetic analysis. We assess how domain organization of the most abundant type of BGCs present among the isolates (Non-ribosomal peptide synthetases and Polyketide synthases) may influence the diversity of compounds produced by these organisms and highlight for the first time the potential for novel compound production from this genus of bacteria, using a genome guided approach.	en
dc.description.sponsorship	Department of Agriculture, Food and the Marine (FIRM 1/F009/MabS, FIRM 13/F/516); Marine Institute (National Development Plan; Sea Change Strategy and the Strategy for Science Technology and Innovation; Beaufort award (C2CRA 2007/082)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	1494
dc.identifier.citation	Naughton, L. M., Romano, S., O’Gara, F. and Dobson, A. D. W. (2017) 'Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds', Frontiers in Microbiology, 8, 1494 (15pp). doi: 10.3389/fmicb.2017.01494	en
dc.identifier.doi	10.3389/fmicb.2017.01494
dc.identifier.issn	1664-302X
dc.identifier.journaltitle	Frontiers in Microbiology	en
dc.identifier.uri	https://hdl.handle.net/10468/4801
dc.identifier.volume	8
dc.language.iso	en	en
dc.publisher	Frontiers Media S.A.	en
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/264266/EU/Towards a Latin America & Caribbean Knowledge Based Bio-Economy (KBBE) in partnership with Europe/ALCUE-KBBE
dc.relation.uri	http://journal.frontiersin.org/article/10.3389/fmicb.2017.01494/full
dc.rights	© 2017, Naughton, Romano, O’Gara and Dobson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Symbiont	en
dc.subject	PKS	en
dc.subject	NRPS	en
dc.subject	Antibiotic resistance	en
dc.subject	Bioactive compounds	en
dc.subject	Nonribosomal peptide synthetases	en
dc.subject	Marine natural products	en
dc.subject	Neighbor joining method	en
dc.subject	Polyketide synthases	en
dc.subject	Colibactin biosynthesis	en
dc.subject	Sp nov	en
dc.subject	Sponges	en
dc.subject	Discovery	en
dc.subject	Bacteria	en
dc.subject	Actinomycete	en
dc.title	Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds	en
dc.type	Article (peer-reviewed)	en