Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2

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dc.contributor.authorAprea, Giuseppe
dc.contributor.authorMullan, William Michael
dc.contributor.authorMurru, Nicoletta
dc.contributor.authorFitzgerald, Gerald F.
dc.contributor.authorBuonanno, Marialuisa
dc.contributor.authorCortesi, Maria Luisa
dc.contributor.authorPrencipe, Vincenza Annunziata
dc.contributor.authorMigliorat, Giacomo
dc.date.accessioned2018-02-06T13:36:28Z
dc.date.available2018-02-06T13:36:28Z
dc.date.issued2017
dc.description.abstractThis work investigated bacteriophage induced starter failures in artisanal buffalo Mozzarella production plants in Southern Italy. Two hundred and ten samples of whey starter cultures were screened for bacteriophage infection. Multiplex polymerase chain reaction (PCR) revealed phage infection in 28.56% of samples, all showing acidification problems during cheese making. Based on DNA sequences, bacteriophages for Lactococcus lactis (L. lactis), Lactobacillus delbruekii (L. delbruekii) and Streptococcus thermophilus (S. thermophilus) were detected. Two phages active against L.  lactis, фApr-1 and фApr-2, were isolated and characterised. The genomes, approximately 31.4 kb and 31 kb for фApr-1 and фApr-2 respectively, consisted of double-stranded linear DNA with pac-type system. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed one major structural protein of approximately 32.5 kDa and several minor proteins. This is the first report of phage isolation in buffalo milk and of the use of multiplex PCR to screen and study the diversity of phages against Lactic Acid Bacteria (LAB) strains in artisanal Water Buffalo Mozzarella starters.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAprea, G., Mullan, W. M., Murru, N., Fitzgerald, G., Buonanno, M., Cortesi, M. L., Prencipe, V. A. and Migliorati, G. (2017) 'Multiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2', Veterinaria Italiana, 53(3), pp. 207-214. doi: 10.12834/VetIt.315.1238.3en
dc.identifier.doi10.12834/VetIt.315.1238.3
dc.identifier.endpage214
dc.identifier.issn0505-401X
dc.identifier.issued3
dc.identifier.journaltitleVeterinaria Italianaen
dc.identifier.startpage207
dc.identifier.urihttps://hdl.handle.net/10468/5393
dc.identifier.volume53
dc.language.isoenen
dc.publisherIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporaleen
dc.relation.urihttps://www.researchgate.net/profile/Giuseppe_Aprea2/publication/320551161_Multiplex_PCR_to_detect_bacteriophages_from_natural_whey_cultures_of_buffalo_milk_and_characterisation_of_two_phages_active_against_Lactococcus_lactis_phApr-1_and_phApr-2/links/5a156b984585150052133d8e/Multiplex-PCR-to-detect-bacteriophages-from-natural-whey-cultures-of-buffalo-milk-and-characterisation-of-two-phages-active-against-Lactococcus-lactis-phApr-1-and-phApr-2.pdf
dc.rights© 2017, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise. Open Access:This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectBacteriophageen
dc.subjectBuffaloen
dc.subjectLactic Acid Bacteria (LAB)en
dc.subjectLactococcus lactisen
dc.subjectMilken
dc.subjectNatural starter cultureen
dc.subjectPCRen
dc.titleMultiplex PCR to detect bacteriophages from natural whey cultures of buffalo milk and characterisation of two phages active against Lactococcus lactis, фApr-1 and фApr-2en
dc.typeArticle (peer-reviewed)en
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