Access to this article is restricted until 12 months after publication by the request of the publisher.. Restriction lift date: 2018-01-20
Direct and rapid electrochemical detection of Pseudomonas aeruginosa quorum signaling molecules in bacterial cultures and cystic fibrosis sputum samples through cationic surfactant-assisted membrane disruption
dc.check.date | 2018-01-20 | |
dc.check.info | Access to this article is restricted until 12 months after publication by the request of the publisher. | en |
dc.contributor.author | Buzid, Alyah | |
dc.contributor.author | Reen, F. Jerry | |
dc.contributor.author | Langsi, Victor K. | |
dc.contributor.author | Ó Muimhneacháin, Eoin | |
dc.contributor.author | O'Gara, Fergal | |
dc.contributor.author | McGlacken, Gerard P. | |
dc.contributor.author | Luong, John H. T. | |
dc.contributor.author | Glennon, Jeremy D. | |
dc.contributor.funder | Science Foundation Ireland | en |
dc.contributor.funder | Seventh Framework Programme | en |
dc.contributor.funder | European Commission | en |
dc.contributor.funder | Department of Agriculture, Food and the Marine | en |
dc.contributor.funder | Irish Research Council for Science, Engineering and Technology | en |
dc.contributor.funder | Horizon 2020 | en |
dc.contributor.funder | Irish Research Council | en |
dc.contributor.funder | Health Research Board | en |
dc.contributor.funder | Marine Institute | en |
dc.contributor.funder | Teagasc | en |
dc.contributor.funder | University College Cork | en |
dc.date.accessioned | 2017-02-02T15:06:20Z | |
dc.date.available | 2017-02-02T15:06:20Z | |
dc.date.issued | 2017-01-20 | |
dc.date.updated | 2017-02-02T14:31:39Z | |
dc.description.abstract | Rapid detection of pathogenic bacteria present in patient samples is of utmost importance for the clinical management of bacterial-induced diseases. Herein, we describe an efficient and direct electrochemical approach for the detection of 2-heptyl-3-hydroxy-4-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ), and pyocyanin (PYO) as a molecular signature of Pseudomonas aeruginosa (PA), a frequently infecting pathogen with high antibiotic resistance. The cationic surfactant hexadecyltrimethylammonium bromide (CTAB) enhances the effectiveness of an unmodified thin-film boron-doped diamond (BDD) electrode for the direct detection of PYO, HHQ, and PQS in bacterial cultures of PAO1 and PA14. Differential pulse voltammetry (DPV) is then used to monitor the production of these microbial metabolites in bacterial cultures of PAO1 over 10 h without any sample pretreatment. A proposed mechanism for the interaction of CTAB with bacteria cells is examined by zeta (ζ) potential measurements. Furthermore, the detection method is successfully extended to a clinical fluid matrix and applied to PA spiked cystic fibrosis (CF) sputum samples. | en |
dc.description.sponsorship | Science Foundation Ireland ((SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438), SFI/EI Technology Innovation Development Award (TIDA) (SFI/12/TIDA/B2405); (SFI/12/IP/1315, SFI/09/RFP/CHS2353, and SSPC2 12/RC/2275)); Department of Agriculture and Food, Ireland (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council for Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647); Irish Research Council (GOIPG/2013/336); Health Research Board/Irish Thoracic Society (MRCG-2014-6); Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013); University College Cork (UCC Strategic Research Fund Ph.D. Studentship.) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Accepted Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | Buzid, A., Reen, F. J., Langsi, V. K., Muimhneacháin, E. Ó., O'Gara, F., McGlacken, G. P., Luong, J. H. T. and Glennon, J. D. (2017) 'Direct and Rapid Electrochemical Detection of Pseudomonas aeruginosa Quorum Signaling Molecules in Bacterial Cultures and Cystic Fibrosis Sputum Samples through Cationic Surfactant-Assisted Membrane Disruption', ChemElectroChem, Article in Press. doi:10.1002/celc.201600590 | en |
dc.identifier.doi | 10.1002/celc.201600590 | |
dc.identifier.issn | 2196-0216 | |
dc.identifier.journaltitle | ChemElectroChem | en |
dc.identifier.uri | https://hdl.handle.net/10468/3555 | |
dc.language.iso | en | en |
dc.publisher | Wiley | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3 | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATION | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE | en |
dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/14/TIDA/2438/IE/Next Generation Antibiotics: anti-biofilm, anti-pathogenic natural bioactives from marine microorganisms./ | |
dc.rights | © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Buzid et al (2017) ChemElectroChem, which has been published in final form at http://dx.doi.org/10.1002/celc.201600590. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms | en |
dc.subject | Cystic fibrosis | en |
dc.subject | Electrochemical detection | en |
dc.subject | Membrane disruption | en |
dc.subject | Pseudomonas aeruginosa | en |
dc.subject | Surfactant | en |
dc.title | Direct and rapid electrochemical detection of Pseudomonas aeruginosa quorum signaling molecules in bacterial cultures and cystic fibrosis sputum samples through cationic surfactant-assisted membrane disruption | en |
dc.type | Article (peer-reviewed) | en |