Direct and rapid electrochemical detection of Pseudomonas aeruginosa quorum signaling molecules in bacterial cultures and cystic fibrosis sputum samples through cationic surfactant-assisted membrane disruption

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dc.contributor.authorBuzid, Alyah
dc.contributor.authorReen, F. Jerry
dc.contributor.authorLangsi, Victor K.
dc.contributor.authorÓ Muimhneacháin, Eoin
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorMcGlacken, Gerard P.
dc.contributor.authorLuong, John H. T.
dc.contributor.authorGlennon, Jeremy D.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderSeventh Framework Programmeen
dc.contributor.funderEuropean Commissionen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderIrish Research Council for Science, Engineering and Technologyen
dc.contributor.funderHorizon 2020en
dc.contributor.funderIrish Research Councilen
dc.contributor.funderHealth Research Boarden
dc.contributor.funderMarine Instituteen
dc.contributor.funderTeagascen
dc.contributor.funderUniversity College Corken
dc.date.accessioned2017-02-02T15:06:20Z
dc.date.available2017-02-02T15:06:20Z
dc.date.issued2017-01-20
dc.date.updated2017-02-02T14:31:39Z
dc.description.abstractRapid detection of pathogenic bacteria present in patient samples is of utmost importance for the clinical management of bacterial-induced diseases. Herein, we describe an efficient and direct electrochemical approach for the detection of 2-heptyl-3-hydroxy-4-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ), and pyocyanin (PYO) as a molecular signature of Pseudomonas aeruginosa (PA), a frequently infecting pathogen with high antibiotic resistance. The cationic surfactant hexadecyltrimethylammonium bromide (CTAB) enhances the effectiveness of an unmodified thin-film boron-doped diamond (BDD) electrode for the direct detection of PYO, HHQ, and PQS in bacterial cultures of PAO1 and PA14. Differential pulse voltammetry (DPV) is then used to monitor the production of these microbial metabolites in bacterial cultures of PAO1 over 10 h without any sample pretreatment. A proposed mechanism for the interaction of CTAB with bacteria cells is examined by zeta (ζ) potential measurements. Furthermore, the detection method is successfully extended to a clinical fluid matrix and applied to PA spiked cystic fibrosis (CF) sputum samples.en
dc.description.sponsorshipScience Foundation Ireland ((SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438), SFI/EI Technology Innovation Development Award (TIDA) (SFI/12/TIDA/B2405); (SFI/12/IP/1315, SFI/09/RFP/CHS2353, and SSPC2 12/RC/2275)); Department of Agriculture and Food, Ireland (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council for Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647); Irish Research Council (GOIPG/2013/336); Health Research Board/Irish Thoracic Society (MRCG-2014-6); Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013); University College Cork (UCC Strategic Research Fund Ph.D. Studentship.)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBuzid, A., Reen, F. J., Langsi, V. K., Muimhneacháin, E. Ó., O'Gara, F., McGlacken, G. P., Luong, J. H. T. and Glennon, J. D. (2017) 'Direct and Rapid Electrochemical Detection of Pseudomonas aeruginosa Quorum Signaling Molecules in Bacterial Cultures and Cystic Fibrosis Sputum Samples through Cationic Surfactant-Assisted Membrane Disruption', ChemElectroChem, Article in Press. doi:10.1002/celc.201600590en
dc.identifier.doi10.1002/celc.201600590
dc.identifier.issn2196-0216
dc.identifier.journaltitleChemElectroChemen
dc.identifier.urihttps://hdl.handle.net/10468/3555
dc.language.isoenen
dc.publisherWileyen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATIONen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAINen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEAen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBAen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMAREen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/14/TIDA/2438/IE/Next Generation Antibiotics: anti-biofilm, anti-pathogenic natural bioactives from marine microorganisms./
dc.rights© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Buzid et al (2017) ChemElectroChem, which has been published in final form at http://dx.doi.org/10.1002/celc.201600590. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. http://olabout.wiley.com/WileyCDA/Section/id-820227.html#termsen
dc.subjectCystic fibrosisen
dc.subjectElectrochemical detectionen
dc.subjectMembrane disruptionen
dc.subjectPseudomonas aeruginosaen
dc.subjectSurfactanten
dc.titleDirect and rapid electrochemical detection of Pseudomonas aeruginosa quorum signaling molecules in bacterial cultures and cystic fibrosis sputum samples through cationic surfactant-assisted membrane disruptionen
dc.typeArticle (peer-reviewed)en
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