Redox proteomic insights into involvement of clathrin-mediated endocytosis in silver nanoparticles toxicity to Mytilus galloprovincialis

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dc.contributor.authorBouallegui, Younes
dc.contributor.authorBen Younes, Ridha
dc.contributor.authorOueslati, Ridha
dc.contributor.authorSheehan, David
dc.date.accessioned2019-09-04T08:36:14Z
dc.date.available2019-09-04T08:36:14Z
dc.date.issued2018-10-29
dc.description.abstractClathrin-mediated endocytosis is a major mode of nanoparticle (NP) internalization into cells. However, influence of internalization routes on nanoparticle toxicity is poorly understood. Here, we assess the impact of blocking clathrin-mediated endocytosis upon silver NP (AgNP) toxicity to gills and digestive glands of the mussel Mytilusgalloprovincialisusing the uptake inhibitor, amantadine. Animals were exposed for 12h to AgNP (< 50 nm) in the presence and absence of amantadine. Labeling of oxidative protein modifications, either thiol oxidation, carbonyl formation or both in two-dimensional electrophoresis separations revealed 16 differentially affected abundance spots. Amongst these, twelve hypothetical proteins were successfully identified by peptide mass fingerprinting (MALDI TOF-MS/MS). The proteins identified are involved in buffering redox status or in cytoprotection. We conclude that blockade of clathrin-mediated endocytosis protected against NP toxicity, suggesting this uptake pathway facilitates toxicity. Lysosomal degradation and autophagy are major mechanisms that might be induced to mitigate NP toxicity.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0205765en
dc.identifier.citationBouallegui, Y., Younes, R.B., Oueslati, R. and Sheehan, D., 2018. Redox proteomic insights into involvement of clathrin-mediated endocytosis in silver nanoparticles toxicity to Mytilus galloprovincialis. PloS one, 13(10), (e0205765). DOI:10.1371/journal.pone.0205765en
dc.identifier.doi10.1371/journal.pone.0205765en
dc.identifier.eissn1932-6203
dc.identifier.endpage15en
dc.identifier.issn1932-6203
dc.identifier.issued10en
dc.identifier.journaltitlePLOS Oneen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8450
dc.identifier.volume13en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205765
dc.rights© 2018 Bouallegui et alen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectClathrin-mediated endocytosisen
dc.subjectNanoparticle (NP)en
dc.subjectMytilusgalloprovincialisusingen
dc.subjectLysosomal degradationen
dc.subjectAutophagyen
dc.titleRedox proteomic insights into involvement of clathrin-mediated endocytosis in silver nanoparticles toxicity to Mytilus galloprovincialisen
dc.typeArticle (peer-reviewed)en
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