Implementation of transposon mutagenesis in Bifidobacterium

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dc.contributor.authorRuiz, Lorena
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.editorRicke, Steven C.
dc.contributor.editorPark, Si Hong
dc.contributor.editorDavis, Morgan L.
dc.date.accessioned2019-07-08T12:05:00Z
dc.date.available2019-07-08T12:05:00Z
dc.date.issued14/06/2019
dc.date.updated2019-07-08T11:45:30Z
dc.description.abstractRandom transposon mutagenesis allows for relatively rapid, genome-wide surveys to detect genes involved in functional traits, by performing screens of mutant libraries. This approach has been widely applied to identify genes responsible for activities of interest in multiple eukaryote and prokaryote organisms, although most studies on microorganisms have focused on pathogenic and clinically relevant bacteria. In this chapter we describe the implementation of an in vitro Tn5-based transposome strategy to generate a large collection of random mutants in the gut commensal Bifidobacterium breve UCC2003, and discuss considerations when applying this mutagenesis system to other Bifidobacterium species or strains of interest.en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRuiz, L. and van Sinderen, D. (2019) 'Implementation of transposon mutagenesis in Bifidobacterium', in Ricke, S. C., Park, S. H. and Davis, M. L. (eds.) Microbial Transposon Mutagenesis: Protocols and Applications. Methods in Molecular Biology, vol. 2016. New York, Humana, pp. 51-62. doi: 10.1007/978-1-4939-9570-7_5en
dc.identifier.doi10.1007/978-1-4939-9570-7_5en
dc.identifier.endpage62en
dc.identifier.isbn978-1-4939-9570-7
dc.identifier.journaltitleMethods in Molecular Biologyen
dc.identifier.startpage51en
dc.identifier.urihttps://hdl.handle.net/10468/8122
dc.identifier.volume2016en
dc.language.isoenen
dc.publisherHumana, New York, NYen
dc.relation.ispartofMicrobial Transposon Mutagenesis: Protocols and Applications
dc.relation.ispartofMethods in Molecular Biology book series (MIMB, volume 2016)
dc.rights© 2019, Springer Science+Business Media, LLC, part of Springer Nature. This is a post-peer-review, pre-copyedit version of a chapter published in Ricke, S. C., Park, S. H. and Davis, M. L. (eds.) Microbial Transposon Mutagenesis: Protocols and Applications. Methods in Molecular Biology, vol. 2016. New York, Humana, pp. 51-62. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9570-7_5en
dc.subjectTn5en
dc.subjectTransposonen
dc.subjectBifidobacteriumen
dc.titleImplementation of transposon mutagenesis in Bifidobacteriumen
dc.typeBook chapteren
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