Direct correlation between potentiometric and impedance biosensing of antibody-antigen interactions using an integrated system

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dc.check.date2018-08-16
dc.check.infoAccess to this article is restricted for 12 months after publication by request of the publisher.en
dc.contributor.authorTsai, Meng-Yen
dc.contributor.authorCreedon, Niamh
dc.contributor.authorBrightbill, Eleanor
dc.contributor.authorPavlidis, Spyridon
dc.contributor.authorBrown, Billyde
dc.contributor.authorGray, Darren W.
dc.contributor.authorShields, Niall
dc.contributor.authorSayers, Rí­ona
dc.contributor.authorMooney, Mark H.
dc.contributor.authorO'Riordan, Alan
dc.contributor.authorVogel, Eric M.
dc.contributor.funderNational Science Foundationen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderDepartment for Employment and Learning, Northern Irelanden
dc.date.accessioned2018-01-03T10:00:11Z
dc.date.available2018-01-03T10:00:11Z
dc.date.issued2017-08-16
dc.date.updated2017-11-24T12:48:09Z
dc.description.abstractA fully integrated system that combines extended gate field-effect transistor (EGFET)-based potentiometric biosensors and electrochemical impedance spectroscopy (EIS)-based biosensors has been demonstrated. This integrated configuration enables the sequential measurement of the same immunological binding event on the same sensing surface and consequently sheds light on the fundamental origins of sensing signals produced by FET and EIS biosensors, as well as the correlation between the two. Detection of both the bovine serum albumin (BSA)/anti-BSA model system in buffer solution and bovine parainfluenza antibodies in complex blood plasma samples was demonstrated using the integrated biosensors. Comparison of the EGFET and EIS sensor responses reveals similar dynamic ranges, while equivalent circuit modeling of the EIS response shows that the commonly reported total impedance change (DZtotal) is dominated by the change in charge transfer resistance (Rct) rather than surface capacitance (Csurface). Using electrochemical kinetics and the Butler-Volmer equation, we unveil that the surface potential and charge transfer resistance, measured by potentiometric and impedance biosensors, respectively, are, in fact, intrinsically linked. This observation suggests that there is no significant gain in using the FET/EIS integrated system and leads to the demonstration that low-cost EGFET biosensors are sufficient as a detection tool to resolve the charge information of biomolecules for practical sensing applications.en
dc.description.sponsorshipNational Science Foundation (CBET Award No. 1264705); Department for Employment and Learning, Northern Ireland (Grant No. USI 039)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid73701
dc.identifier.citationTsai, M.-Y., Creedon, N., Brightbill, E., Pavlidis, S., Brown, B., Gray, D. W., Shields, N., Sayers, R., Mooney, M. H., O'Riordan, A. and Vogel, E. M. (2017) 'Direct correlation between potentiometric and impedance biosensing of antibody-antigen interactions using an integrated system', Applied Physics Letters, 111(7), 73701(5pp). doi:10.1063/1.4986190en
dc.identifier.doi10.1063/1.4986190
dc.identifier.endpage5en
dc.identifier.issn0003-6951
dc.identifier.issn1077-3118
dc.identifier.issued7en
dc.identifier.journaltitleApplied Physics Lettersen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/5211
dc.identifier.volume111en
dc.language.isoenen
dc.publisherAIP Publishingen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI US Ireland R&D Partnership/12/US/I2476/IE/Multichannel disposable sensors for animal health disease diagnostics/en
dc.rights© 2017, the Authors. Reproduced from Tsai, M.-Y., Creedon, N., Brightbill, E., Pavlidis, S., Brown, B., Gray, D. W., Shields, N., Sayers, R., Mooney, M. H., O'Riordan, A. and Vogel, E. M. (2017) 'Direct correlation between potentiometric and impedance biosensing of antibody-antigen interactions using an integrated system', Applied Physics Letters, 111(7), 73701(5pp). doi:10.1063/1.4986190, with the permission of AIP Publishing.en
dc.subjectBiochemistryen
dc.subjectBiosensorsen
dc.subjectCapacitanceen
dc.subjectElectrochemical impedance spectroscopyen
dc.subjectElectrochemical sensorsen
dc.subjectMolecular biophysicsen
dc.subjectProteinsen
dc.titleDirect correlation between potentiometric and impedance biosensing of antibody-antigen interactions using an integrated systemen
dc.typeArticle (peer-reviewed)en
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