Insights into the mechanisms of eukaryotic translation gained with ribosome profiling

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dc.contributor.authorAndreev, Dmitry E.
dc.contributor.authorO'Connor, Patrick B. F.
dc.contributor.authorLoughran, Gary
dc.contributor.authorDmitriev, Sergey E.
dc.contributor.authorBaranov, Pavel V.
dc.contributor.authorShatsky, Ivan N.
dc.contributor.funderRussian Science Foundationen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2017-01-04T09:17:57Z
dc.date.available2017-01-04T09:17:57Z
dc.date.issued2016-12-06
dc.date.updated2017-01-03T13:18:41Z
dc.description.abstractThe development of Ribosome Profiling (RiboSeq) has revolutionized functional genomics. RiboSeq is based on capturing and sequencing of the mRNA fragments enclosed within the translating ribosome and it thereby provides a â snapshotâ of ribosome positions at the transcriptome wide level. Although the method is predominantly used for analysis of differential gene expression and discovery of novel translated ORFs, the RiboSeq data can also be a rich source of information about molecular mechanisms of polypeptide synthesis and translational control. This review will focus on how recent findings made with RiboSeq have revealed important details of the molecular mechanisms of translation in eukaryotes. These include mRNA translation sensitivity to drugs affecting translation initiation and elongation, the roles of upstream ORFs in response to stress, the dynamics of elongation and termination as well as details of intrinsic ribosome behavior on the mRNA after translation termination. As the RiboSeq method is still at a relatively early stage we will also discuss the implications of RiboSeq artifacts on data interpretation.en
dc.description.sponsorshipRussian Science Foundation (Grant Numbers 14-14-00127 and RBFR 14-04-00412); Science Foundation Ireland (Grant Number 12/IA/1335)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAndreev, D.E., O'Connor, P.B.F., Loughran, G., Dmitriev, S.E., Baranov, P.V. and Shatsky, I.N. (2016) ‘Insights into the mechanisms of eukaryotic translation gained with ribosome profiling’, Nucleic Acids Research, 45(2), pp. 513-526. doi: 10.1093/nar/gkw1190en
dc.identifier.doi10.1093/nar/gkw1190
dc.identifier.endpage526
dc.identifier.issn0305-1048
dc.identifier.issued2
dc.identifier.journaltitleNucleic Acids Researchen
dc.identifier.startpage513
dc.identifier.urihttps://hdl.handle.net/10468/3416
dc.identifier.volume45
dc.language.isoenen
dc.publisherOxford University Press on behalf of Nucleic Acids Researchen
dc.rights© 2016, the Authors. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectFunctional genomicsen
dc.subjectPolypeptide synthesisen
dc.subjectElongationen
dc.subjectTerminationen
dc.subjectRibosome profilingen
dc.titleInsights into the mechanisms of eukaryotic translation gained with ribosome profilingen
dc.typeArticle (peer-reviewed)en
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