Rab11 family expression in the human placenta: localization at the maternal-fetal interface

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dc.contributor.authorTaglauer, Elizabeth S.
dc.contributor.authorArtemiuk, Patrycja A.
dc.contributor.authorHanscom, Sara R.
dc.contributor.authorLindsay, Andrew J.
dc.contributor.authorWuebbolt, Danielle
dc.contributor.authorBreathnach, Fionnuala M.
dc.contributor.authorTully, Elizabeth C.
dc.contributor.authorKhan, Amir R.
dc.contributor.authorMcCaffrey, Mary W.
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderIrish Research Council for Science, Engineering and Technology
dc.contributor.funderIrish Research Council
dc.date.accessioned2017-10-18T09:40:16Z
dc.date.available2017-10-18T09:40:16Z
dc.date.issued2017
dc.description.abstractRab proteins are a family of small GTPases involved in a variety of cellular processes. The Rab11 subfamily in particular directs key steps of intracellular functions involving vesicle trafficking of the endosomal recycling pathway. This Rab subfamily works through a series of effector proteins including the Rab11-FIPs (Rab11 Family-Interacting Proteins). While the Rab11 subfamily has been well characterized at the cellular level, its function within human organ systems is still being explored. In an effort to further study these proteins, we conducted a preliminary investigation of a subgroup of endosomal Rab proteins in a range of human cell lines by Western blotting. The results from this analysis indicated that Rab11a, Rab11c(Rab25) and Rab14 were expressed in a wide range of cell lines, including the human placental trophoblastic BeWo cell line. These findings encouraged us to further analyse the localization of these Rabs and their common effector protein, the Rab Coupling Protein (RCP), by immunofluorescence microscopy and to extend this work to normal human placental tissue. The placenta is a highly active exchange interface, facilitating transfer between mother and fetus during pregnancy. As Rab11 proteins are closely involved in transcytosis we hypothesized that the placenta would be an interesting human tissue model system for Rab investigation. By immunofluorescence microscopy, Rab11a, Rab11c(Rab25), Rab14 as well as their common FIP effector RCP showed prominent expression in the placental cell lines. We also identified the expression of these proteins in human placental lysates by Western blot analysis. Further, via fluorescent immunohistochemistry, we noted abundant localization of these proteins within key functional areas of primary human placental tissues, namely the outer syncytial layer of placental villous tissue and the endothelia of fetal blood vessels. Overall these findings highlight the expression of the Rab11 family within the human placenta, with novel localization at the maternal-fetal interface.en
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology (Post-Doctoral fellowship GOIPD/2014/42)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0184864
dc.identifier.citationTaglauer, E. S., Artemiuk, P. A., Hanscom, S. R., Lindsay, A. J., Wuebbolt, D., Breathnach, F. M., Tully, E. C., Khan, A. R. and McCaffrey, M. W. (2017) 'Rab11 family expression in the human placenta: Localization at the maternal-fetal interface', PLOS ONE, 12(9), e0184864 (15pp). doi: 10.1371/journal.pone.0184864en
dc.identifier.doi10.1371/journal.pone.0184864
dc.identifier.endpage15
dc.identifier.issn1932-6203
dc.identifier.issued9
dc.identifier.journaltitlePLOS ONEen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4905
dc.identifier.volume12
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IA/1239/IE/Molecular Aspects of Immune Evasion and Subversion of Membrane Trafficking by Pathogens/
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Principal Investigator Programme (PI)/09/IN.1/B2629/IE/Functional analysis of the Rab11 and Rab14 GTPases and their associated proteins in eukaryotic membrane trafficking with potential identification of targets for therapeutic intervention in the treatment of human disease/
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0184864
dc.rights© 2017, Taglauer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPlacentaen
dc.subjectImmunofluorescence microscopyen
dc.subjectBlood vesselsen
dc.subjectProtein expressionen
dc.subjectDAPI stainingen
dc.subjectPregnancyen
dc.subjectTrophoblastsen
dc.subjectVesiclesen
dc.titleRab11 family expression in the human placenta: localization at the maternal-fetal interfaceen
dc.typeArticle (peer-reviewed)en
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