From raw milk cheese to the gut: investigating the colonization strategies of Bifidobacterium mongoliense

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dc.check.date2025-02-16en
dc.check.infoAccess to this article is restricted until 6 months after publication by request of the publisheren
dc.contributor.authorLonghi, Giuliaen
dc.contributor.authorLugli, Gabriele Andreaen
dc.contributor.authorTarracchini, Chiaraen
dc.contributor.authorFontana, Federicoen
dc.contributor.authorBianchi, Massimiliano Giovannien
dc.contributor.authorCarli, Elisaen
dc.contributor.authorBussolati, Ovidioen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.authorTurroni, Francescaen
dc.contributor.authorVentura, Marcoen
dc.contributor.editorDudley, Edward G.en
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderMinistero dell’Istruzione, dell’Università e della Ricercaen
dc.date.accessioned2024-11-12T14:16:39Z
dc.date.available2024-11-12T14:16:39Z
dc.date.issued2024-08-16en
dc.description.abstractThe microbial ecology of raw milk cheeses is determined by bacteria originating from milk and milk-producing animals. Recently, it has been shown that members of the Bifidobacterium mongoliense species may become transmitted along the Parmigiano Reggiano cheese production chain and ultimately may colonize the consumer intestine. However, there is a lack of knowledge regarding the molecular mechanisms that mediate the interaction between B. mongoliense and the human gut. Based on 128 raw milk cheeses collected from different Italian regions, we isolated and characterized 10 B. mongoliense strains. Comparative genomics allowed us to unveil the presence of enzymes required for the degradation of sialylated host-glycans in B. mongoliense, corroborating the appreciable growth on de Man-Rogosa-Sharpe (MRS) medium supplemented with 3’-sialyllactose (3′-SL) or 6’-sialyllactose (6′-SL). The B. mongoliense BMONG18 was chosen, due to its superior ability to utilize 3′-SL and mucin as representative strain, to investigate its behavior when co-inoculated with other bifidobacterial species. Conversely, members of other bifidobacterial species did not appear to benefit from the presence of BMONG18, highlighting a competitive scenario for nutrient acquisition. Transcriptomic data of BMONG18 reveal no significant differences in gene expression when cultivated in a gut simulating medium (GSM), regardless of whether cheese was included or not. Furthermore, BMONG18 was shown to exhibit high adhesion capabilities to HT29-MTX human cells, in line with its colonization ability of a human host.en
dc.description.sponsorshipScience Foundation Ireland (Grant numbers SFI/12/RC/2273a and SFI/12/RC/2273b); Ministero dell’Istruzione, dell’Università e della Ricerca (Project code PE00000003; CUP D93C22000890001)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide01244-24en
dc.identifier.citationLonghi, G., Lugli, G. A., Tarracchini, C., Fontana, F., Bianchi, M. G., Carli, E., Bussolati, O., van Sinderen, D., Turroni, F. and Ventura, M. (2024) 'From raw milk cheese to the gut: investigating the colonization strategies of Bifidobacterium mongoliense', Applied and Environmental Microbiology, 90(9), e01244-24 (20pp). https://doi.org/10.1128/aem.01244-24en
dc.identifier.doihttps://doi.org/10.1128/aem.01244-24en
dc.identifier.eissn1098-5336en
dc.identifier.endpage20en
dc.identifier.issn0099-2240en
dc.identifier.issued9en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/16641
dc.identifier.volume90en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2024, American Society for Microbiology. All Rights Reserved.en
dc.subjectBifidobacteriaen
dc.subjectMicrobiotaen
dc.subjectMicrobiomeen
dc.subjectDairy food microorganismsen
dc.subjectMicrobial genomicsen
dc.subjectMicrobe-host interactionsen
dc.titleFrom raw milk cheese to the gut: investigating the colonization strategies of Bifidobacterium mongolienseen
dc.typeArticle (peer-reviewed)en
oaire.citation.issue9en
oaire.citation.volume90en
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