Mutations in the intersubunit bridge regions of 16S rRNA affect decoding and subunit–subunit interactions on the 70S ribosome

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dc.contributor.authorSun, Qing
dc.contributor.authorVila-Sanjurjo, Anton
dc.contributor.authorO'Connor, Michael
dc.contributor.funderUniversity of Missouri-Kansas City
dc.contributor.funderUniversity College Cork
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderNational Science Foundation
dc.date.accessioned2017-11-14T13:24:31Z
dc.date.available2017-11-14T13:24:31Z
dc.date.issued2011
dc.description.abstractThe small and large subunits of the ribosome are held together by a series of bridges, involving RNA-RNA, RNA-protein and protein-protein interactions. Some 12 bridges have been described for the Escherichia coli 70S ribosome. In this work, we have targeted for mutagenesis, some of the 16S rRNA residues involved in the formation of intersubunit bridges B3, B5, B6, B7b and B8. In addition to effects on subunit association, the mutant ribosomes also affect the fidelity of translation; bridges B5, B6 and B8 increase decoding errors during elongation, while disruption of bridges B3 and B7b alters the stringency of start codon selection. Moreover, mutations in the bridge B5, B6 and B8 regions of 16S rRNA also correct the growth and decoding defects associated with alterations in ribosomal protein S12. These results link bridges B5, B6 and B8 with the decoding process and are consistent with the recently described location of translation factor EF-Tu on the ribosome and the proposed involvement of h14 in activating Guanosine-5'-triphosphate (GTP) hydrolysis by aminoacyl-tRNA center dot EF-Tu center dot GTP. These observations are consistent with a model in which bridges B5, B6 and B8 contribute to the fidelity of translation by modulating GTP hydrolysis by aminoacyl-tRNA center dot EF-Tu center dot GTP teRNAry complexes during the elongation phase of protein synthesis.en
dc.description.sponsorshipNational Science Foundation (MCB0745025)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSun, Q., Vila-Sanjurjo, A. and O’Connor, M. (2011) 'Mutations in the intersubunit bridge regions of 16S rRNA affect decoding and subunit–subunit interactions on the 70S ribosome', Nucleic Acids Research, 39(8), pp. 3321-3330. doi: 10.1093/nar/gkq1253en
dc.identifier.doi10.1093/nar/gkq1253
dc.identifier.endpage3330
dc.identifier.issn0305-1048
dc.identifier.issued8
dc.identifier.journaltitleNucleic Acids Researchen
dc.identifier.startpage3321
dc.identifier.urihttps://hdl.handle.net/10468/5026
dc.identifier.volume39
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkq1253
dc.rights© 2010, the Authors. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.5
dc.subjectAminoacyl-transfer-RNAen
dc.subjectA-site fingeren
dc.subjectEscherichia-colien
dc.subjectSalmonella-typhimuriumen
dc.subjectTranslation initiationen
dc.subjectAntibiotic resistanceen
dc.subjectAngstrom resolutionen
dc.subjectCrystal structureen
dc.subjectEF-Tuen
dc.subjectDiscriminationen
dc.titleMutations in the intersubunit bridge regions of 16S rRNA affect decoding and subunit–subunit interactions on the 70S ribosomeen
dc.typeArticle (peer-reviewed)en
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