Tracking yeast metabolism and the Crabtree effect in real time via CO2 production using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

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dc.contributor.authorAhmed, M. Rizwan
dc.contributor.authorDoyle, Nicholas
dc.contributor.authorConnolly, Cathal
dc.contributor.authorMcSweeney, Seán
dc.contributor.authorKrüse, Jacob
dc.contributor.authorMorrissey, John P.
dc.contributor.authorPrentice, Michael B.
dc.contributor.authorFitzpatrick, Dara
dc.contributor.funderAlltechen
dc.contributor.funderIrish Research Councilen
dc.date.accessioned2021-12-10T15:19:34Z
dc.date.available2021-12-10T15:19:34Z
dc.date.issued2019-11-30
dc.description.abstractIn this study, a new approach to measure metabolic activity of yeast via the Crabtree effect is described. BARDS is an analytical technique developed to aid powder and tablet characterisation by monitoring changes in the compressibility of a solvent during solute dissolution. It is a rapid and simple method which utilises a magnetic stir bar to mix added solute and induce the acoustic resonance of a vessel containing a fixed volume of solvent. In this study it is shown that initiation of fermentation in a yeast suspension, in aqueous buffer, is accompanied by reproducible changes in the frequency of induced acoustic resonance. These changes signify increased com-pressibility of the suspension due to CO2release by the yeast. A simple standardised BARDS protocol reveals yeast carbon source preferences and can generate quantitative kinetic data on carbon source metabolism which are characteristic of each yeast strain. The Crawford-Woods equation can be used to quantify total gaseous CO2produced by a given number of viable yeast when supplied with a fixed amount of carbon source. This allows for a value to be calculated for the amount of gaseous CO2produced by each yeast cell. The approach has the potential to transform the way in which yeast metabolism is tracked and potentially provide an orthogonal or surrogate method to determining viability, vitality and attenuation measurements in the future.en
dc.description.sponsorshipIrish Research Council (Grant no. IRC/EPSPG/2014/84)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAhmed, M. R., Doyle, N., Connolly, C., McSweeney, S., Krüse, J., Morrissey, J. P., Prentice, M. B. and Fitzpatrick, D. (2020) ‘Tracking yeast metabolism and the Crabtree effect in real time via CO2 production using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)', Journal of Biotechnology, 308, pp. 63-73. doi:10.1016/j.jbiotec.2019.11.016en
dc.identifier.doi10.1016/j.jbiotec.2019.11.016en
dc.identifier.endpage73en
dc.identifier.issn0168-1656
dc.identifier.journaltitleJournal of Biotechnologyen
dc.identifier.startpage63en
dc.identifier.urihttps://hdl.handle.net/10468/12349
dc.identifier.volume308en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.rights© 2019 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 licenseen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectYeasten
dc.subjectSpectroscopyen
dc.subjectAcousticsen
dc.subjectCrabtree effecten
dc.subjectMetabolismen
dc.subjectViability and vitalityen
dc.titleTracking yeast metabolism and the Crabtree effect in real time via CO2 production using Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)en
dc.typeArticle (peer-reviewed)en
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