dc.contributor.author	Carrión, Víctor J.
dc.contributor.author	van der Voort, Menno
dc.contributor.author	Arrebola, Eva
dc.contributor.author	Gutiérrez-Barranquero, José A.
dc.contributor.author	de Vicente, Antonio
dc.contributor.author	Raaijmakers, Jos M.
dc.contributor.author	Cazorla, Francisco M.
dc.contributor.funder	Regional Government of Andalusia, Spain	en
dc.contributor.funder	Junta de Andalucía (CICE), Spain	es
dc.contributor.funder	Ministerio de Ciencia e Innovación, Spain	es
dc.contributor.funder	European Commission
dc.contributor.funder	University of Málaga, Spain
dc.contributor.funder	Consejo Superior de Investigaciones Científicas, Spain
dc.date.accessioned	2016-02-02T16:12:27Z
dc.date.available	2016-02-02T16:12:27Z
dc.date.issued	2014-02-21
dc.description.abstract	Background: The antimetabolite mangotoxin is a key factor in virulence of Pseudomonas syringae pv. Syringae strains which cause apical necrosis of mango trees. Previous studies showed that mangotoxin biosynthesis is governed by the mbo operon. Random mutagenesis led to the identification of two other gene clusters that affect mangotoxin biosynthesis. These are the gacS/gacA genes and mgo operon which harbors the four genes mgoBCAD. Results: The current study shows that disruption of the nonribosomal peptide synthetase (NRPS) gene mgoA resulted in loss of mangotoxin production and reduced virulence on tomato leaves. Transcriptional analyses by qPCR and promoter reporter fusions revealed that mbo expression is regulated by both gacS/gacA and mgo genes. Also, expression of the mgo operon was shown to be regulated by gacS/gacA. Heterologous expression under the native promoter of the mbo operon resulted in mangotoxin production in non-producing P. syringae strains, but not in other Pseudomonas species. Also introduction of the mbo and mgo operons in nonproducing P. protegens Pf-5 did not confer mangotoxin production but did enhance transcription of the mbo promoter. Conclusions: From the data obtained in this study, we conclude that both mbo and mgo operons are under the control of the gacS/gacA two-component system and that the MgoA product acts as a positive regulator of mangotoxin biosynthesis.	en
dc.description.sponsorship	Junta de Andalucía (CICE), Spain (Ayudas Grupo PAIDI AGR-169; Proyecto de Excelencia P07-AGR-02471; fellowship to Víctor J. Carrión); Ministerio de Ciencia e Innovacion, Spain (Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica AGL2011-30354-C02-01); European Commission (FEDER; European Social Fund); University of Málaga, Spain (Plan Propio); Consejo Superior de Investigaciones Científicas, Spain (JAEDoc grant to Eva Arrebola)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	46
dc.identifier.citation	CARRIÓN, V. J., VAN DER VOORT, M., ARREBOLA, E., GUTIÉRREZ-BARRANQUERO, J. A., DE VICENTE, A., RAAIJMAKERS, J. M. & CAZORLA, F. M. 2014. Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA. BMC Microbiology, 14:46, 1-13. http://dx.doi.org/10.1186/1471-2180-14-46	en
dc.identifier.doi	10.1186/1471-2180-14-46
dc.identifier.endpage	13	en
dc.identifier.issn	1471-2180
dc.identifier.journaltitle	BMC Microbiology	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/2243
dc.identifier.volume	14	en
dc.language.iso	en	en
dc.publisher	BioMed Central Ltd.	en
dc.rights	© Carrión et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.	en
dc.rights.uri	http://creativecommons.org/licenses/by/2.0	en
dc.subject	Plant-microbe interaction	en
dc.subject	Antimetabolite toxin	en
dc.subject	Mgo operon	en
dc.subject	GacS/GacA	en
dc.title	Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA	en
dc.type	Article (peer-reviewed)	en

Mangotoxin production of Pseudomonas syringae pv. syringae is regulated by MgoA

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