Clinical applications of contact spectroscopy in the intra-operative detection of fluorescence in high grade glioma

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dc.contributor.advisorRedmond, Henry Paul
dc.contributor.advisorexternalO'Sullivan, Michael Gerard Josephen
dc.contributor.authorDablouk, Mohamed Osman Faisalen
dc.contributor.funderStrykeren
dc.date.accessioned2026-05-19T13:56:39Z
dc.date.available2026-05-19T13:56:39Z
dc.date.issued2024-11-30
dc.date.submitted2024-11-30
dc.description.abstractFluorescence-guided surgery is known to improve rates of complete resection in high grade glioma, translating to an improvement in progression-free survival. The conventional technique which utilises microscope widefield blue-light fluorescence, although beneficial, is associated with some drawbacks which may be addressed through utilisation of alternative contact-based techniques. Contact-based spectroscopy is helpful for detecting protoporphyrin IX (PpIX) fluorescence, but this technique has not been validated for clinical use. In this work, we describe a series of clinical research experiments whereby the use of an optical fibre probe for the detection of PpIX is investigated. In chapter 2, a prospective comparative study demonstrates that the microscope-based technique identified PpIX fluorescence with an accuracy of 61%, while the optical probe was 74% accurate. In Chapter 3, the characteristics of the probe are investigated in a cadaveric animal model, followed by in vivo use to demonstrate the practicality of the probe. Here, we demonstrate that use of the probe is not associated with an increase in post-operative complications. Finally, chapter 4 describes the design of a machine-learning algorithm which aims to determine IDH status and MGMT methylation status based on spectroscopic features. Here, we describe how a prediction model may be able to determine IDH status but is unreliable for predicting MGMT methylation status. In conclusion, optical fibre spectroscopy demonstrates an improved level of accuracy compared to the conventional microscope-based technique, is not associated with intra- or post-operative complications, and may offer the ability to determine certain molecular characteristics of tumour tissue in real time.en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDablouk, M. O. F. 2024. Clinical applications of contact spectroscopy in the intra-operative detection of fluorescence in high grade glioma. MD Thesis, University College Cork.en
dc.identifier.endpage61en
dc.identifier.urihttps://hdl.handle.net/10468/18784
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2024, Mohamed Osman Dablouk.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectGliomaen
dc.subjectGlioblastomaen
dc.subjectFluorescenceen
dc.subject5-ALAen
dc.subjectSpectroscopyen
dc.titleClinical applications of contact spectroscopy in the intra-operative detection of fluorescence in high grade glioma
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnameMD - Doctor of Medicineen
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