ODX: a fitness tracker-based device for continuous bacterial growth monitoring

Yallapragada, V. V. B.; Gowda, Uday; Wong, David; O'Faolain, Liam; Tangney, Mark; Devarapu, Ganga Chinna Rao

ODX: a fitness tracker-based device for continuous bacterial growth monitoring

dc.check.date	2020-09-03
dc.check.info	Access to this article is restricted until 12 months after publication by request of the publisher.	en
dc.contributor.author	Yallapragada, V. V. B.
dc.contributor.author	Gowda, Uday
dc.contributor.author	Wong, David
dc.contributor.author	O'Faolain, Liam
dc.contributor.author	Tangney, Mark
dc.contributor.author	Devarapu, Ganga Chinna Rao
dc.contributor.funder	Horizon 2020	en
dc.contributor.funder	Health Research Board	en
dc.contributor.funder	Breakthrough Cancer Research	en
dc.date.accessioned	2020-07-29T12:13:25Z
dc.date.available	2020-07-29T12:13:25Z
dc.date.issued	2019-09-03
dc.description.abstract	Continuous monitoring of bacterial growth in aqueous media is a crucial process in academic research as well as in the biotechnology industry. Bacterial growth is usually monitored by measuring the optical density of bacteria in liquid media, using benchtop spectrophotometers. Due to the large form factor of the existing spectrophotometers, they cannot be used for live monitoring of the bacteria inside bacterial incubation chambers. Additionally, the use of benchtop spectrometers for continuous monitoring requires multiple samplings and is labor intensive. To overcome these challenges, we have developed an optical density measuring device (ODX) by modifying a generic fitness tracker. The resulting ODX device is an ultraportable and low-cost device that can be used inside bacterial incubators for real-time monitoring even while shaking is in progress. We evaluated the performance of ODX with different bacterial types and growth conditions and compared it with a commercial benchtop spectrophotometer. In all cases, ODX showed comparable performance to that of the standard benchtop spectrophotometer. Finally, we demonstrate a simple and useful smartphone application whereby the user is notified when the bacterial concentration reaches the targeted value. Due to its potential for automation and mass production, we believe that the ODX has a wide range of applications in biotechnology research and industry.	en
dc.description.sponsorship	Health Research Board (Grant MRCG2016-25)	en
dc.description.status	Peer reviewed	en
dc.description.version	Accepted Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Yallapragada, V. V. B., Gowda, U., Wong, D., O’Faolain, L., Tangney, M. and Devarapu, G. C. R. (2019) 'ODX: a fitness tracker-based device for continuous bacterial growth monitoring', Analytical Chemistry, 91(19), pp. 12329–12335. doi: 10.1021/acs.analchem.9b02628	en
dc.identifier.doi	10.1021/acs.analchem.9b02628	en
dc.identifier.endpage	12335	en
dc.identifier.issn	1520-6882
dc.identifier.issued	19	en
dc.identifier.journaltitle	Analytical Chemistry	en
dc.identifier.startpage	12329	en
dc.identifier.uri	https://hdl.handle.net/10468/10328
dc.identifier.volume	91	en
dc.language.iso	en	en
dc.publisher	ACS Publications	en
dc.relation.project	info:eu-repo/grantAgreement/EC/H2020::MSCA-COFUND-FP/713654/EU/Career Development Fellowships in the National Technology Centre Programme/Career-FIT	en
dc.rights	© 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry after technical editing by the publisher. To access the final edited and published work see https://pubs-acs-org.ucc.idm.oclc.org/doi/10.1021/acs.analchem.9b02628#	en
dc.subject	Sensors	en
dc.subject	Bacteria	en
dc.subject	Circuits	en
dc.subject	3D printing	en
dc.subject	Diodes	en
dc.title	ODX: a fitness tracker-based device for continuous bacterial growth monitoring	en
dc.type	Article (peer-reviewed)	en