Electrochemical detection and capillary electrophoresis: Comparative studies for alkaline phosphatase (ALP) release from living cells

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dc.contributor.authorBalbaied, Thanih
dc.contributor.authorHogan, Anna
dc.contributor.authorMoore, Eric
dc.contributor.funderMinistry of Education – Kingdom of Saudi Arabien
dc.date.accessioned2020-11-13T15:59:48Z
dc.date.available2020-11-13T15:59:48Z
dc.date.issued2020-08-11
dc.date.updated2020-11-13T15:47:19Z
dc.description.abstractAlkaline phosphatase (ALP) is one of the main biomarkers that is clinically detected in bone and liver disorders using optical assays. The electrochemical principle is important because point-of-care testing is increasing dramatically and absorbance techniques hardly compete with the medical revolution that is occurring. The detection of ALP using electrochemical detection is contributing to the integration systems field, and hence enhancing the detection of biological targets for pharmaceutical research and design systems. Moreover, in vitro electrochemical measurements use cost effective materials and simple techniques. Graphite screen-printed electrodes and linear sweep voltammetry were used to optimize the electrochemistry of the enzymatic product p-aminophenol using the enzyme kinetic assay. ALP release from embryonic and cancer cells was determined from adhesion cell culture. Additionally, capillary electrophoresis and colorimetric methods were applied for comparison assays. The resulting assays showed a dynamic range of ALP ranging from 1.5 to 1500 U/L, and limit of detection of 0.043 U/L. This was achieved by using 70 μL of the sample and an incubation time of 10 min at an optimal substrate concentration of 9.6 mM of p-aminophenol phosphate. A significant difference (p < 0.05) was measured between the absorbance assays. This paper demonstrates the advantages of the electrochemical assay for ALP release from cells, which is in line with recent trends in gene expression systems using microelectrode array technologies and devices for monitoring electrophysiological activity.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid95en
dc.identifier.citationBalbaied, T., Hogan, A. and Moore, E. (2020) 'Electrochemical Detection and Capillary Electrophoresis: Comparative Studies for Alkaline Phosphatase (ALP) Release from Living Cells', Biosensors, 10(8), 95, (14 pp). doi: 10.3390/bios10080095en
dc.identifier.doi10.3390/bios10080095en
dc.identifier.endpage14en
dc.identifier.issn2079-6374
dc.identifier.issued8en
dc.identifier.journaltitleBiosensorsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/10754
dc.identifier.volume10en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.mdpi.com/2079-6374/10/8/95
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectElectrochemistryen
dc.subjectLinear sweep voltammetryen
dc.subjectAlkaline phosphataseen
dc.subjectCapillary electrophoresisen
dc.subjectColorimetryen
dc.subjectCancer cellsen
dc.subjectEmbryonic cellsen
dc.titleElectrochemical detection and capillary electrophoresis: Comparative studies for alkaline phosphatase (ALP) release from living cellsen
dc.typeArticle (peer-reviewed)en
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