dc.contributor.author	Huang, Shungen
dc.contributor.author	Li, Yi Ping
dc.contributor.author	Zhang, Qi
dc.contributor.author	Redmond, H. Paul
dc.contributor.author	Wang, Jiang Huai
dc.contributor.author	Wang, Jian
dc.contributor.funder	Natural Science Foundation of Jiangsu Province, China
dc.contributor.funder	Innovative Team of Jiangsu Province, China
dc.contributor.funder	Jiangsu Province Program of Innovative and Entrepreneurial Talents, China
dc.date.accessioned	2016-02-25T17:21:53Z
dc.date.available	2016-02-25T17:21:53Z
dc.date.issued	2013-06-20
dc.description.abstract	Background: Surgical intervention-related trauma contributes largely to the development of postoperative immunosuppression, with reduced resistance to secondary bacterial infection. This study compared the impact of laparotomy versus laparoscopy on macrophage-associated bactericidal ability and examined whether laparotomy renders the host more susceptible to microbial infection. Results: BALB/c mice were randomized into control, laparotomy, and laparoscopy groups. Laparotomy, but not laparoscopy, significantly downregulated CR3 expression on macrophages, diminished macrophage-induced uptake and phagocytosis of E. coli and S. aureus, and impaired macrophage-mediated intracellular bacterial killing. Consistent with this, mice that underwent laparotomy displayed substantially higher bacterial counts in the blood and visceral organs as well as a significantly enhanced mortality rate following bacterial infection, whereas mice subjected to laparoscopy did not show any defects in their bacterial clearance. Conclusion: Laparotomy has an adverse effect on host innate immunity against microbial infection by impairing macrophage-mediated phagocytosis and killing of the invaded bacteria. By contrast, laparoscopy appears to preserve macrophage-associated bactericidal ability, thus alleviating the development of postoperative immunosuppression.	en
dc.description.sponsorship	Natural Science Foundation of Jiangsu Province, China (BK2011310); Innovative Team of Jiangsu Province, China (LJ201141); Jiangsu Province Program of Innovative and Entrepreneurial Talents, China (2011–2014)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	27
dc.identifier.citation	HUANG, S. G., LI, Y. P., ZHANG, Q., REDMOND, H. P., WANG, J. H. & WANG, J. 2013. Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model. BMC Immunology, 14:27, 1-8. http://dx.doi.org/10.1186/1471-2172-14-27	en
dc.identifier.doi	10.1186/1471-2172-14-27
dc.identifier.endpage	8	en
dc.identifier.issn	1471-2172
dc.identifier.journaltitle	BMC Immunology	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/2404
dc.identifier.volume	14	en
dc.language.iso	en	en
dc.publisher	BioMed Central Ltd.	en
dc.rights	© Huang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.	en
dc.rights.uri	http://creativecommons.org/licenses/by/2.0	en
dc.subject	Laparotomy	en
dc.subject	Laparoscopy	en
dc.subject	Phagocytosis	en
dc.subject	Bactericidal activity	en
dc.subject	Innate immunity	en
dc.subject	Macrophages	en
dc.title	Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model	en
dc.type	Article (peer-reviewed)	en

Laparotomy and laparoscopy diversely affect macrophage-associated antimicrobial activity in a murine model

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