dc.contributor.author	Zhang, Yuanbao
dc.contributor.author	Wei, Chao
dc.contributor.author	Jiang, Wendi
dc.contributor.author	Wang, Lei
dc.contributor.author	Li, Churui
dc.contributor.author	Wang, Yunyue
dc.contributor.author	Dow, J. Maxwell
dc.contributor.author	Sun, Wenxian
dc.date.accessioned	2016-02-17T11:46:19Z
dc.date.available	2016-02-17T11:46:19Z
dc.date.issued	2013
dc.description.abstract	Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most important diseases in rice. However, little is known about the pathogenicity mechanisms of Xoc. Here we have investigated the function of three HD-GYP domain regulatory proteins in biofilm formation, the synthesis of virulence factors and virulence of Xoc. Deletion of rpfG resulted in altered production of extracellular polysaccharides (EPS), abolished virulence on rice and enhanced biofilm formation, but had little effect on the secretion of proteases and motility. In contrast, mutational analysis showed that the other two HD-GYP domain proteins had no effect on virulence factor synthesis and tested phenotypes. Mutation of rpfG led to up-regulation of the type III secretion system and altered expression of three putative glycosyltransferase genes gumD, pgaC and xagB, which are part of operons directing the synthesis of different extracellular polysaccharides. The pgaABCD and xagABCD operons were greatly up-regulated in the Xoc Delta rpfG mutant, whereas the expression of the gum genes was unaltered or slightly enhanced. The elevated biofilm formation of the Xoc Delta rpfG mutant was dramatically reduced upon deletion of gumD, xagA and xagB, but not when pgaA and pgaC were deleted. Interestingly, only the Delta gumD mutant, among these single gene mutants, exhibits multiple phenotype alterations including reduced biofilm and EPS production and attenuated virulence on rice. These data indicate that RpfG is a global regulator that controls biofilm formation, EPS production and bacterial virulence in Xoc and that both gumD- and xagB-dependent EPS contribute to biofilm formation under different conditions.	en
dc.description.sponsorship	973 program (2011CB100700); National Natural Science Foundation of China (NSFC) (31272007); PCSIRT Program (IRT1042, NCET-09-0736); Ph.D. Programs Foundation of Ministry of Education of China	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e59428
dc.identifier.citation	Zhang Y, Wei C, Jiang W, Wang L, Li C, Wang Y, et al. (2013) The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice. PLoS ONE 8(3): e59428. doi:10.1371/journal.pone.0059428	en
dc.identifier.doi	10.1371/journal.pone.0059428
dc.identifier.issn	1932-6203
dc.identifier.issued	3	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2386
dc.identifier.volume	8	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2013 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Diffusible Signal Factor	en
dc.subject	Di-Gmp Turnover	en
dc.subject	Pseudomonas-Aeruginosa	en
dc.subject	2-Component System	en
dc.subject	Gene-Expression	en
dc.subject	Campestris	en
dc.subject	Vesicatoria	en
dc.subject	Phosphodiesterase	en
dc.subject	Pathogenicity	en
dc.subject	Sequences	en
dc.title	The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice	en
dc.type	Article (peer-reviewed)	en

The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice

Files

Original bundle

Collections