Development of PLGA nanoparticle loaded dissolving microneedles and comparison with hollow microneedles in intradermal vaccine delivery

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dc.contributor.authorMönkäre, Juha
dc.contributor.authorPontier, Maria
dc.contributor.authorvan Kampen, Eveline E. M.
dc.contributor.authorDu, Guangsheng
dc.contributor.authorLeone, Mara
dc.contributor.authorRomeijn, Stefan
dc.contributor.authorNejadnik, M. Reza
dc.contributor.authorO'Mahony, Conor
dc.contributor.authorSlütter, Bram
dc.contributor.authorJiskoot, Wim
dc.contributor.authorBouwstra, Joke A.
dc.contributor.funderSeventh Framework Programmeen
dc.contributor.funderEuropean Federation of Pharmaceutical Industries and Associationsen
dc.date.accessioned2019-10-02T04:38:38Z
dc.date.available2019-10-02T04:38:38Z
dc.date.issued2018-05-24
dc.description.abstractSkin is an attractive but also very challenging immunisation site for particulate subunit vaccines. The aim of this study was to develop hyaluronan (HA)-based dissolving microneedles (MNs) loaded with PLGA nanoparticles (NPs) co-encapsulating ovalbumin (OVA) and poly(I:C) for intradermal immunisation. The NP:HA ratio used for the preparation of dissolving MNs appeared to be critical for the quality of MNs and their dissolution in ex vivo human skin. Asymmetrical flow field-flow fractionation and dynamic light scattering were used to analyse the NPs released from the MNs in vitro. Successful release of the NPs depended on the drying conditions during MN preparation. The delivered antigen dose from dissolving MNs in mice was determined to be 1 µg OVA, in NPs or as free antigen, by using near-infrared fluorescence imaging. Finally, the immunogenicity of the NPs after administration of dissolving MNs (NP:HA weight ratio 1:4) was compared with that of hollow MN-delivered NPs in mice. Immunization with free antigen in dissolving MNs resulted in equally strong immune responses compared to delivery by hollow MNs. However, humoral and cellular immune responses evoked by NP-loaded dissolving MNs were inferior to those elicited by NPs delivered through a hollow MN. In conclusion, we identified several critical formulation parameters for the further development of NP-loaded dissolving MNs.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMönkäre, J., Pontier, M., van Kampen, E.E., Du, G., Leone, M., Romeijn, S., Nejadnik, M.R., O'Mahony, C., Slütter, B., Jiskoot, W. and Bouwstra, J.A., 2018. Development of PLGA nanoparticle loaded dissolving microneedles and comparison with hollow microneedles in intradermal vaccine delivery. European Journal of Pharmaceutics and Biopharmaceutics, 129, (11pp). DOI:10.1016/j.ejpb.2018.05.031en
dc.identifier.doi10.1016/j.ejpb.2018.05.031en
dc.identifier.eissn1873-3441
dc.identifier.endpage121en
dc.identifier.issn0939-6411
dc.identifier.journaltitleEuropean Journal of Pharmaceutics and Biopharmaceuticsen
dc.identifier.startpage111en
dc.identifier.urihttps://hdl.handle.net/10468/8664
dc.identifier.volume129en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::SP1-JTI/115363/EU/Collaboration on the Optimisation of Macromolecular Pharmaceutical Access to Cellular Targets/COMPACTen
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0939641118303412
dc.rights© 2018 The Authors. Published by Elsevier B.V.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectDissolving microneedlesen
dc.subjectSkin immunisationen
dc.subjectVaccine deliveryen
dc.subjectParticulate vaccinesen
dc.subjectPLGA nanoparticlesen
dc.titleDevelopment of PLGA nanoparticle loaded dissolving microneedles and comparison with hollow microneedles in intradermal vaccine deliveryen
dc.typeArticle (peer-reviewed)en
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