Impact of the defined Oligo-MM12 microbiota on intestinal colonisation and dissemination of Listeria monocytogenes

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dc.contributor.authorCazzaniga, Monica
dc.contributor.authorBra, Kardokh Kaka
dc.contributor.authorHerzog, Mathias K. M.
dc.contributor.authorHardt, Wolf-Dietrich
dc.contributor.authorClaesson, Marcus J.
dc.contributor.authorMathur, Harsh
dc.contributor.authorGahan, Cormac G. M.
dc.contributor.funderHorizon 2020 Framework Programme
dc.contributor.funderSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNSF
dc.contributor.funderNCCR Catalysis, NCCR
dc.contributor.funderH2020 Marie Skłodowska-Curie Actions, AMSC, 956279
dc.contributor.funderScience Foundation Ireland, SFI, SFI/12/RC/2273_P2
dc.date.accessioned2026-07-02T09:50:05Z
dc.date.available2026-07-02T09:50:05Z
dc.date.issued2026-03-09
dc.description.abstractListeria monocytogenes is a foodborne pathogen of global concern, particularly for immunocompromised individuals at risk of severe disease. In mice, infection outcomes are strongly influenced by host immunity and gut microbiome composition. The Oligo-MM12 defined microbiota mouse model, containing a simplified community of 12 bacterial strains, offers a controlled system to study L. monocytogenes pathogenesis and microbiome interactions. Defined or reduced-complexity microbiota models are increasingly used to investigate colonisation resistance and identify protective taxa. In this study, we compared Oligo-MM12 mice with conventionally raised Specific Pathogen Free (SPF) mice to assess how microbiome complexity shapes infection. This allowed us to explore how microbiome complexity affects resistance to L. monocytogenes. We performed an in vivo infection study to assess host responses and pathogen-related outcomes, alongside an ex vivo fermentation assay that simulated the murine distal colon, to monitor microbial dynamics. Building on our earlier work, we now demonstrate that in vivo, Oligo-MM12 mice showed significantly higher L. monocytogenes shedding in faeces during infection, whereas SPF mice progressively reduced L. monocytogenes levels. Despite this, L. monocytogenes dissemination to internal organs after three days of infection was similar in both models. Alterations to gut Prevotella, Akkermansia and Blautia species following L. monocytogenes infection were noteworthy. Ex vivo fermentation mirrored in vivo patterns, validating the Oligo-MM12 system for mechanistic studies. Together, these results highlight the importance of microbiome complexity in modulating infection outcomes and establish a foundation for identifying protective taxa and mechanisms of colonization resistance.en
dc.description.sponsorshipThis project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 956279. We acknowledge funding from Science Foundation Ireland (grant number SFI/12/RC/2273_P2). W.D.H. was supported in part by the NCCR Microbiomes, a program funded by the Swiss National Science foundation.
dc.description.versionPublished Version
dc.format.extent15
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid8434
dc.identifier.authororcidCazzaniga, Monica
dc.identifier.authororcidBra, Kardokh Kaka
dc.identifier.authororcidHerzog, Mathias K. M.
dc.identifier.authororcidHardt, Wolf-Dietrich
dc.identifier.authororcidClaesson, Marcus J.§0000-0002-5712-0623
dc.identifier.authororcidMathur, Harsh
dc.identifier.authororcidGahan, Cormac G. M.§0000-0003-2385-8192
dc.identifier.citationCazzaniga, M, Bra, K K, Herzog, M K M, Hardt, W-D, Claesson, M J, Mathur, H & Gahan, C G M 2026, 'Impact of the defined Oligo-MM12 microbiota on intestinal colonisation and dissemination of Listeria monocytogenes', Scientific Reports, vol. 16, no. 1, 8434, pp. 1-15. https://doi.org/10.1038/s41598-026-37294-4
dc.identifier.doi10.1038/s41598-026-37294-4
dc.identifier.endpage15
dc.identifier.issn2045-2322
dc.identifier.issued1
dc.identifier.journaltitleScientific Reports
dc.identifier.otherPubMedCentral: PMC12972299
dc.identifier.otherORCID: /0000-0002-5712-0623/work/219530354
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/18991
dc.identifier.volume16
dc.language.isoen
dc.publisherNature Research
dc.relation.urihttps://www.scopus.com/pages/publications/105032897806
dc.rights© 2026, the Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
dc.rights.accessrightsopen access
dc.rights.licensenameAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.statusPeer reviewed
dc.subjectAnimals
dc.subjectDisease models, animal
dc.subjectFeces/microbiology
dc.subjectFemale
dc.subjectGastrointestinal microbiome
dc.subjectListeria monocytogenes/pathogenicity
dc.subjectListeriosis/microbiology
dc.subjectMice
dc.subjectMice, inbred C57BL
dc.subjectSpecific pathogen-free organisms
dc.subject[Microbiology]
dc.subjectmicro-Matrix bioreactor
dc.subjectListeria monocytogenes
dc.subjectSPF
dc.subjectGut Microbiome
dc.subjectOligo-MM
dc.titleImpact of the defined Oligo-MM12 microbiota on intestinal colonisation and dissemination of Listeria monocytogenesen
dc.typeArticle (peer-reviewed)
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